ChemicalBook > CAS DataBase List > EC 3.4.21.19

EC 3.4.21.19

Product Name
EC 3.4.21.19
CAS No.
66676-43-5
Chemical Name
EC 3.4.21.19
Synonyms
Glu-C;PROTEASE V8;V8 PROTEASE;EC 3.4.21.19;Proteinase V8;V8 proteinase;Glu-C, 500 u/mg;V8 endoprotease;proteinase V8 VAN;PROTEASE, S AUREUS
CBNumber
CB2117702
Molecular Formula
NULL
Formula Weight
0
MOL File
Mol file
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EC 3.4.21.19 Property

storage temp. 
-20°C
form 
lyophilized powder
color 
light brown
biological source
Staphylococcus aureus (V8)
Water Solubility 
Soluble in water
Specific Activity
500-1,000units/mg solid
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Safety

Hazard Codes 
Xn
Risk Statements 
36/37/38-42/43
Safety Statements 
22-24-26-36/37-45
WGK Germany 
3
RTECS 
TW0175000
10
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H317May cause an allergic skin reaction

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
P2922
Product name
Endoproteinase Glu-C from Staphylococcus aureus V8
Purity
Type XVII-B, lyophilized powder, 500-1,000?units/mg solid
Packaging
100units
Price
$58.8
Updated
2025/07/31
Sigma-Aldrich
Product number
P2922
Product name
Endoproteinase Glu-C from Staphylococcus aureus V8
Purity
Type XVII-B, lyophilized powder, 500-1,000?units/mg solid
Packaging
500units
Price
$198
Updated
2025/07/31
Sigma-Aldrich
Product number
P6181
Product name
Endoproteinase Glu-C from Staphylococcus aureus V8
Purity
suitable for protein sequencing, lyophilized powder
Packaging
50μg
Price
$246
Updated
2025/07/31
Sigma-Aldrich
Product number
P2922
Product name
Endoproteinase Glu-C from Staphylococcus aureus V8
Purity
Type XVII-B, lyophilized powder, 500-1,000?units/mg solid
Packaging
2500Units
Price
$682
Updated
2025/07/31
Sigma-Aldrich
Product number
ENDOGLUS-RO
Product name
Endoproteinase Glu-C Sequencing Grade
Purity
from Staphylococcus aureus V8
Packaging
50μg
Price
$235
Updated
2025/07/31
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EC 3.4.21.19 Chemical Properties,Usage,Production

Uses

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Product P2922 has been used to linearize cyclic peptides in C. ternatea leaf extract.

Uses

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:

  • obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
  • the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
  • limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
  • the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.

Biochem/physiol Actions

Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

EC 3.4.21.19 Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from EC 3.4.21.19 manufacturers

Career Henan Chemical Co
Product
EC 3.4.21.19 66676-43-5
Price
US $1.00/KG
Min. Order
1KG
Purity
99.0%
Supply Ability
100kg
Release date
2020-01-14

66676-43-5, EC 3.4.21.19Related Search:


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  • 66676-43-5
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