4,5-DIAMINOFLUORESCEIN

Product Name: 4,5-DIAMINOFLUORESCEIN
CAS No.: 205391-01-1
Chemical Name: 4,5-DIAMINOFLUORESCEIN
Synonyms: DAF-2;5-Diaminofluorescein;4,5-DIAMINOFLUORESCEIN;5,6-DIAMINOFLUORESCEIN;DAF-2;4;5-DIAMINOFLUORESCEIN;4,5-Diaminofluorescein, DAF-2;DAF-2, Fluorescent nitric oxide probe;4,5-Diaminofluorescein-Isopropanol adduct (1:2);2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID;5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
CBNumber: CB3354894
Molecular Formula: C20H14N2O5
Formula Weight: 362.34
MOL File: 205391-01-1.mol

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4,5-DIAMINOFLUORESCEIN Property

storage temp.: 2-8°C
form: powder
color: Brown to black

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Safety

Hazard Codes: Xn,Xi
Risk Statements: 22-36/37/38
Safety Statements: 23-26-36
WGK Germany: 3
F: 8-10-23

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P330Rinse mouth.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 251500-M
Product name: DAF-2-CAS205391-01-1-Calbiochem
Purity: Ahighlysensitiveandspecificfluorescentindicatorforthedirectdetectiono
Packaging: 1mg
Price: $610
Updated: 2025/07/31

Sigma-Aldrich

Product number: 251500-M
Product name: DAF-2 - CAS 205391-01-1 - Calbiochem
Purity: A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Packaging: 251500-1MG
Price: $591
Updated: 2024/03/01

Cayman Chemical

Product number: 85160
Product name: DAF-2
Purity: ≥98%
Packaging: 100μg
Price: $61
Updated: 2024/03/01

Cayman Chemical

Product number: 85160
Product name: DAF-2
Purity: ≥98%
Packaging: 250μg
Price: $140
Updated: 2024/03/01

Sigma-Aldrich

Product number: D224
Product name: 4,5-Diaminofluorescein
Purity: 5 mM in DMSO, ≥97% (HPLC)
Packaging: 1mg
Price: $723
Updated: 2025/07/31

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4,5-DIAMINOFLUORESCEIN Chemical Properties,Usage,Production

Description

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM.

Uses

DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.

Uses

Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.

References

[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI: 10.1248/cpb.46.373
[2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI: 10.1016/s0014-5793(98)00440-2

4,5-DIAMINOFLUORESCEIN Preparation Products And Raw materials

Raw materials

Preparation Products

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4,5-DIAMINOFLUORESCEIN Suppliers

China 81 India 1 Switzerland 3 United Kingdom 4 United States 8 Global 97

BOC Sciences

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View Lastest Price from 4,5-DIAMINOFLUORESCEIN manufacturers

Xian confluore Biological Technology Co., Ltd.

Product: DAF-2 205391-01-1
Price: US $0.00/mg
Min. Order: 5mg
Purity: >99.00%
Supply Ability: 5mg
Release date: 2024-03-28

205391-01-1, 4,5-DIAMINOFLUORESCEINRelated Search:

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2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID

4,5-DIAMINOFLUORESCEIN

DAF-2

5,6-DIAMINOFLUORESCEIN

4,5-Diaminofluorescein, DAF-2

4,5-Diaminofluorescein-Isopropanol adduct (1:2)

5-Diaminofluorescein

DAF-2;4;5-DIAMINOFLUORESCEIN

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5,6-diamino-3',6'-dihydroxy-

5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one

DAF-2, Fluorescent nitric oxide probe

205391-01-1

C20H14N2O5

C20H14N2O52C3H8O