iodoHoechst 33258
- Product Name
- iodoHoechst 33258
- CAS No.
- 158013-42-4
- Chemical Name
- iodoHoechst 33258
- Synonyms
- iodoHoechst 33258;Meta-iodoHoechst 33258;2,6'-Bi-1H-benzimidazole, 2'-(3-iodophenyl)-6-(4-methyl-1-piperazinyl)-
- CBNumber
- CB62666657
- Molecular Formula
- C25H23IN6
- Formula Weight
- 534.39
- MOL File
- 158013-42-4.mol
iodoHoechst 33258 Property
- Boiling point:
- 752.5±70.0 °C(Predicted)
- Density
- 1.587±0.06 g/cm3(Predicted)
- solubility
- 25℃: DMSO or water Protect from light
- form
- Powder
- pka
- 10.66±0.10(Predicted)
- color
- Off-white to pink
- Appearance
- Solid Powder
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H302Harmful if swallowed
- Precautionary statements
-
P280Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- DYE0015124
- Product name
- META-IODOHOECHST 33258
- Purity
- 95.00%
- Packaging
- 5MG
- Price
- $496.98
- Updated
- 2021/12/16
iodoHoechst 33258 Chemical Properties,Usage,Production
Description
Meta-iodoHoechst 33258, an iodinated DNA-binding bibenzimidazole, is a cell dye for DNA quantitation which sensitizes DNA and cells to UVA.
Uses
meta-iodoHoechst 33258 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].
References
[1] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. DOI:10.1016/0167-4781(88)90079-6
[2] Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. DOI:10.1177/23.7.1095650
[3] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
iodoHoechst 33258 Preparation Products And Raw materials
Raw materials
Preparation Products
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