ChemicalBook > CAS DataBase List > 6-Thioguanine

6-Thioguanine

Product Name
6-Thioguanine
CAS No.
154-42-7
Chemical Name
6-Thioguanine
Synonyms
THIOGUANINE;tioguanine;6-tg;2-AMINO-6-PURINETHIOL;lanvis;6-MERCAPTOGUANINE;2-AMINO-6-MERCAPTOPURINE;2-Amino-3,7-dihydropurine-6-thione;2-Amino-3,7-dihydro-6H-purine-6-thione;6H-Purine-6-thione, 2-amino-1,9-dihydro-
CBNumber
CB6361837
Molecular Formula
C5H5N5S
Formula Weight
167.19
MOL File
154-42-7.mol
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6-Thioguanine Property

Melting point:
≥300 °C(lit.)
Density 
1.483 (estimate)
refractive index 
1.5605 (estimate)
storage temp. 
2-8°C
solubility 
Practically insoluble in water and in ethanol (96%). It dissolves in dilute solutions of alkali hydroxides.
Boiling point:
555.4±42.0 °C(Predicted)
form 
lyophilized powder
pka
pKa 8.22 (Uncertain)
color 
Yellow to green
biological source
synthetic (organic)
Water Solubility 
soluble
Merck 
14,9337
BRN 
157765
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMF may be stored at -20°C for up to 1 month.
CAS DataBase Reference
154-42-7(CAS DataBase Reference)
EPA Substance Registry System
Thioguanine (154-42-7)
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Safety

Hazard Codes 
T,Xi
Risk Statements 
25-23/24/25
Safety Statements 
28-36/37/39-45-28A
RIDADR 
UN 2811 6.1/PG 3
WGK Germany 
3
RTECS 
UP0740000
F 
13
Hazard Note 
Irritant
HazardClass 
6.1
PackingGroup 
III
HS Code 
29335990
Hazardous Substances Data
154-42-7(Hazardous Substances Data)
Toxicity
LD50 oral in mouse: 160mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H301Toxic if swalloed

Precautionary statements

P301+P310IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
PHR3250
Product name
Thioguanine
Purity
pharmaceutical secondary standard, certified reference material
Packaging
500MG
Price
$207
Updated
2025/07/31
Sigma-Aldrich
Product number
BP878
Product name
Tioguanine
Purity
British Pharmacopoeia (BP) Reference Standard
Packaging
100MG
Price
$276
Updated
2025/07/31
Sigma-Aldrich
Product number
A4660
Product name
6-Thioguanine
Purity
Hybri-Max , 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
Packaging
2.5mg
Price
$27.4
Updated
2025/07/31
Sigma-Aldrich
Product number
1660000
Product name
Thioguanine
Purity
United States Pharmacopeia (USP) Reference Standard
Packaging
200mg
Price
$380
Updated
2025/07/31
TCI Chemical
Product number
T0212
Product name
6-Thioguanine
Purity
>95.0%(HPLC)
Packaging
1g
Price
$94
Updated
2025/07/31
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6-Thioguanine Chemical Properties,Usage,Production

Description

6-Thioguanine (NSC 752, Thioguanine, 2-Amino-6-purinethiol), is an antineoplastic metabolic antagonist that inhibits DNA synthesis by being metabolically converted to 6-thioGMP. This inhibits purine biosynthesis at multiple steps, and may be phosphorylated and incorporated into DNA. In humans, it causes bone marrow depression and gastrointestinal toxicity. Due to safety problems it is currently less used as an antineoplastic agent but has some use as therapy for ulcerative colitis.

Chemical Properties

Crystalline, lyophilized, sterile, endot. It is white or slightly yellow powder with umami taste, easily soluble in water, but insoluble in organic solvents such as ethanol and acetone.

Originator

Thioguanine,Burroughs-Wellcome,US,1966

Uses

6-Thioguanine acts as an antineoplastic and purine antimetabolite. It is also useful as an inhibition of stimulated expression of TNF-related apoptosis-inducing ligand (TRAIL) protein. It is involved in the treatment of acute leukemias and Psoriasis. Further, it is used for the treatment of ulcerative colitis and autoimmune diseases.

Definition

ChEBI: 6-Thioguanine is a 2-aminopurine that is the 6-thiono derivative of 2-amino-1,9-dihydro-6H-purine.

Indications

6-Thioguanine is a purine analogue structurally related to 6-mercaptopurine and azathioprine. Thioguanine interferes with several enzymes required for de novo purine synthesis, and its metabolites are incorporated into DNA and RNA, further impeding nucleic acid synthesis. The mechanism of action of thioguanine in psoriasis is not clearly understood; it has been hypothesized to affect the proliferation and trafficking of lymphocytes as well as the proliferation of keratinocytes.

Application

6-Thioguanine is a variant of guanine with hydrogen bonding at the N-7 of the purine ring. Its association with cytosine alters the dimension of the base stacking. 6-Thioguanine usage in treating inflammatory bowel disease (IBD) contributes to nodular regenerative hyperplasia (NRH) in the liver.
6-Thioguanine has been used:
to induce autophagy and apoptosis in colorectal cancer cell lines HCT116
as a selection marker in the mutation and survival assay in chinese hamster lung fibroblasts culture V79
as a selection marker in clonogenic Lung metastasis assay of 4T1-luc cells

Manufacturing Process

A mixture of 2.7 grams of finely divided guanine, 10 grams of pulverized phosphorus pentasulfide, 10 ml of pyridine and 100 ml of tetralin was heated at 200°C with mechanical stirring for 5 hours. After cooling, the mixture was filtered and the insoluble residue treated with 150 ml of water and 50 ml of concentrated ammonium hydroxide. The ammoniacal solution was filtered, heated to boiling and acidified with acetic acid. Upon cooling, 2-amino-6- mercaptopurine precipitated as a dark yellow powder, according to US Patent 2,697,709.

brand name

Tioguanine is INN and BAN.

Therapeutic Function

Cancer chemotherapy

General Description

6-thioguanine appears as odorless or almost odorless pale yellow crystalline powder. The drug is available in 40-mg tablets for oral use. It is used to treat acute nonlymphocytic leukemia.

Air & Water Reactions

6-Thioguanine may be sensitive to prolonged exposure to air. Insoluble in water.

Reactivity Profile

6-Thioguanine is incompatible with strong oxidizing agents.

Fire Hazard

Flash point data for 6-Thioguanine are not available; however, 6-Thioguanine is probably combustible.

Biochem/physiol Actions

Ribosylated and phosphorylated by the same pathway as natural purine bases; as the nucleotide, inhibits a variety of cellular processes involved in nucleic acid synthesis. Has a long history as an effective treatment of leukemia.

Mechanism of action

The mechanism of action involves incorporation of thetriphosphate into DNA and RNA, resulting in inhibition ofprocessing and function. Thioguanine is a purine antagonist. It is a pro-drug that is converted intracellullarly directly to thioguanine monophosphate (also called 6-thioguanylic acid) (TGMP) by the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). TGMP is further converted to the di- and triphosphates, thioguanosine diphosphate (TGDP) and thioguanosine triphosphate (TGTP). The cytotoxic effect of thioguanine is a result of the incorporation of these nucleotides into DNA. Thioguanine has some immunosuppressive activity.1 Thioguanine is specific for the S phase of the cell cycle.

Side effects

Dose-related myelosuppression is the major adverse effect produced by 6-thioguanine. Patients deficient in thiopurine methyltransferase (TPMT), a cytosolic enzyme required for metabolism of 6-thioguanine, are at heightened risk. Other adverse effects include gastrointestinal complaints and elevations of liver transaminases. There have been rare reports of more serious hepatotoxicity, including acute hepatitis, acute cholestasis, and hepatic venoocclusive disease.

Safety Profile

Poison by ingestion andintraperitoneal routes. Human mutation data reported. Anexperimental teratogen. Other reproductive effects. Ahuman skin irritant. When heated to decomposition itemits very toxic fumes of SOx and NOx.

Synthesis

Thioguanine, 2-aminopurin-6-thiol (30.1.2.12), is made from 2,8-dichloro- 6-hydroxypurine (30.1.2.7), in which the second chlorine atom at C2 is replaced with an amino group when reacted with ammonia, forming 2-amino-8-chloro-6-hydroxy-purine (30.1.2.7), which is then reduced by hydrogen iodide to 2-aminopurin-6-ol (30.1.2.11). Replacement of the hydroxyl group with a mercapto group at C6 is carried out by reacting it with phosphorous pentasulfide, which forms thioguanine (30.1.2.12).

Veterinary Drugs and Treatments

Thioguanine may be useful as adjunctive therapy for acute lymphocytic or granulocytic leukemia in dogs or cats.

Drug interactions

Potentially hazardous interactions with other drugs
Antipsychotics: avoid concomitant use with clozapine (increased risk of agranulocytosis).

Metabolism

Tioguanine undergoes extensive metabolism in the liver and other tissues to several active and inactive metabolites. Tioguanine is inactivated mainly by methylation to aminomethylthiopurine; small amounts are deaminated to thioxanthine, and may go on to be oxidised by xanthine oxidase to thiouric acid, but inactivation is essentially independent of xanthine oxidase and is not affected by inhibition of the enzyme. 24-46
% of the dose is excreted in the urine within 24 hours. It is excreted in the urine almost entirely as metabolites

storage

Room temperature

Purification Methods

It crystallises from H2O as needles. It has UV at 258 and 347nm (H2O, pH 1) and 242, 270 and 322nm max (H2O, pH 11). [Elion & Hitchings J Am Chem Soc 77 1676 1955, Fox et al. J Am Chem Soc 80 1669 1958.] It is an antineoplastic agent [Kataoka et al. Cancer Res 44 519 1984]. [Beilstein 26 III/IV 3926.]

References

1) Wang and Wang (2009), 6-thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo; Biochemistry, 48 2290
2) Yuan et al. (2011), 6-thioguanine reactivates epigenetically silenced genes in acute lymphoblastic leukemia cells by facilitating proteasome-mediated degradation of DNMT1; Cancer Res., 71 1904
3) Bohon and de los Santos (2005), Effect of 6-thioguanine on the stability of duplex DNA; Nucleic Acid Res., 33 2880
4) Issaeva et al. (2010), 6-thioguanine selectively kills BRCA2-defective tumors and overcomes PARP inhibitor resistance; Cancer Res., 70 6268

6-Thioguanine Preparation Products And Raw materials

Raw materials

Butyric anhydride Ethanol 5'—NUCLEOTIDE Guanine Phosphodiesterase I 6-Chloroguanine Ribonucleic acid Phosphorus pentasulfide ANION EXCHANGE RESIN 717 Guanine hydrochloride Polyurethane foam plastic Ion exchange resin column Ethyl acetate Hydrochloric acid Dialysis tubing Acetic acid Sodium hydroxide Anhydrous pyridine Syrup, NF Sodium hydrosulfide Cyclic AMP Industrial ammonia Adenosine 5'-monophosphate PETROLEUM ETHER

Preparation Products

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View Lastest Price from 6-Thioguanine manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
6-Thioguanine 154-42-7
Price
US $0.00/kg
Min. Order
1kg
Purity
98%min
Supply Ability
1000kg
Release date
2023-01-13
Hebei Chuanghai Biotechnology Co., Ltd
Product
6-Thioguanine 154-42-7
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-10-28
Hebei Yanxi Chemical Co., Ltd.
Product
6-Thioguanine 154-42-7
Price
US $70.00-10.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
10 tons
Release date
2023-09-22

154-42-7, 6-ThioguanineRelated Search:

Ancitabine 6-Methylthioguanine 6-Thioguanosine hydrate 1,9-DIHYDRO-6H-PURINE-6-THIONE 6-N-HEPTYLMERCAPTOPURINE 2-Amino-6-mercaptopurine-13C2,15N 6,8-DIHYDROXY-2-METHYLMERCAPTOPURINE 6-(Methylthio)purine 6-thioinosine-5'-triphosphate 8-AZA-6-HYDROXY-2-MERCAPTOPURINE 1,7,8,9-tetrahydro-8-thioxo-6H-purin-6-one 6-METHYLMERCAPTOPURINE RIBOSIDE 2-MERCAPTOPURINE 6-METHYL-D3-MERCAPTOPURINE 2,6-Dithiopurine 1H-Imidazole-4-carboxylic acid 6-thioinosine 5'-monophosphate Thioguanine deoxyriboside,β-Thioguanine deoxyriboside Thioguanine

  • 2-AMINO-6-PURINETHIOL
  • 2-AMINO-6-MERCAPTOPURINE
  • 2-aminopurine-6(1h)-thione
  • 2-amino-purine-6(1h)-thion
  • 2-Amino-6-mercaptopurine, 2-Amino-6-purinethiol
  • 5'-ribonucleotide
  • dibutyryl cyclic AMP calcium salt
  • complex coenzyme
  • 2-Amino-3,7-dihydropurine-6-thione
  • 2-Amino-9H-purine-6(1H)-thione
  • 2-Amino-6-purinethiol ,98%
  • 6-THIOGUANINE (6SG)
  • 2-Amino-6-purinethiol,95%
  • Thioguanine (200 mg)
  • 6-TG/Thioguanine
  • Thioguanine API
  • 2-Amino-6-mercaptopurine 6-Mercaptoguanine
  • 2-amino-1,9-dihydro-6H-purine-6-thione
  • 2-Amino-1,7-dihydro-6H-purin-6-thion [Czech]
  • 2-Amino-6-merkaptopurin [Czech]
  • 2-Aminopurin-6-thiol [Czech]
  • 2-amino-1,7-dihydro-6h-purine-6-thione
  • 2-amino-6-merkaptopurin
  • 2-amino-6-mp
  • 2-aminopurin-6-thiol
  • 6-Thioguanine (6-TG)
  • 2-aMino-1H-purine-6(7H)-thione
  • 2-aMino-3H-purine-6(7H)-thione
  • 2-amino-5,6-dihydro-3H-purine-6-thiol
  • Azathioprine Impurity 6
  • 6H-Purine-6-thione, 2-amino-1,9-dihydro-
  • 2-amino-purine-6-thio
  • 2-aminopurine-6-thiol
  • 6-mercapto-2-aminopurine
  • 6-tg
  • bw5071
  • lanvis
  • nsc-752
  • purine-b-thiol,2-amino
  • tabloid
  • thio-guanin
  • tioguanin
  • tioguanine
  • wellcomeu3b
  • 2-Amino-9H-purine-6-thiol
  • 2-Amino-1,7(9)-dihydro-purine-6-thione
  • 2-amino-6-mercaptopurine (50X)*gamma-irradiated C
  • 2-amino-6-mercaptopurine crystalline
  • 2-Amino-6-purinethiol (6-Thioguanine)
  • 2-AMINO-6-MERCAPTOPURINE (50X)GAMMA-IRRA DIATED CEL
  • Tioguanine(Thioguanine)
  • 6-Thioguanine,~97%
  • 6-THIOGUANIDE
  • 2-AMINO-6-THIOLPURINE
  • 2-amino-6-purinethio
  • 6-THIOGUANINE 97% (HPLC)
  • 2-Thioguanine
  • Guanine, t