1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1) Chemical Properties

solubility 

Soluble in DMSO, DMF, DCM

form 

Solid

color 

Pink to red

Appearance

Dark blue solid

ex/em

680/698 nm (PBS buffer)

ε(extinction coefficient)

198000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.20

1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1) Usage And Synthesis

Description

Cy5.5 azide is a functionalized cyanine dye containing an azide group which can react with alkynes for copper-catalyzed Click Chemistry. Cyanine5.5 is a far-red dye working great for live organism imaging and applications requiring low fluorescence background.

Chemical Properties

Appearance: Dark blue solid
ex/em: 680/698 nm (PBS buffer)
Extinction coefficient (ε): 198000 L??mol??1??cm??1
Quantum yield (Φ): 0.20

Uses

Cyanine 5.5 azide (CY 5.5 azide) is a potent fluorescent dye. Cyanine 5.5 azide can label DNA. Cyanine 5.5 azide can be used for NIR live organism imaging. (λex=684 nm, λem=710 nm)[1][2]. Cyanine 5.5 azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

References

[1] Sun H, et, al. Zwitterionic Cross-Linked Biodegradable Nanocapsules for Cancer Imaging. Langmuir. 2019 Feb 5;35(5):1440-1449. DOI:10.1021/acs.langmuir.8b01633
[2] Damaj MI, et, al. In vivo characterization of a novel inhibitor of CNS nicotinic receptors. Eur J Pharmacol. 2005 Oct 3;521(1-3):43-8. DOI:10.1016/j.ejphar.2005.06.056

1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1)(1557136-25-0)Related Product Information

• diSulfo-Cy5 amine
• Cyanine5.5 tetrazine
• Cy5.5 carboxylic acid
• Sulfo-Cyanine5.5 azide
• Sulfo-Cy5.5 amine
• Cyanine5.5 amine
• Cyanine5.5 maleimide
• diSulfo-Cy5 alkyne
• Cyanine5.5 NHS ester
• 3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt
• 3H-Indolium, 2-[5-[1-(5-carboxypentyl)-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl-5-sulfo-, inner salt, potassium salt (1:1)
• Cy5.5 alkyne
• Sulfo-Cy5 NHS Methyl type | Lumiprobe type
• Cy5 DBCO
• Sulfo Cy5 Maleimide
• Trisulfo-Cy5.5-Alkyne
• Sulfo-Cyanine5 tetrazine