Basic information Safety Supplier Related

Tris(hydroxymethyl)aminomethane acetate salt

Basic information Safety Supplier Related

Tris(hydroxymethyl)aminomethane acetate salt Basic information

Product Name:
Tris(hydroxymethyl)aminomethane acetate salt
Synonyms:
  • TRIS(HYDROXYMETHYL) AMINOMETHANE ACETATE extrapure AR
  • TRIZMA ACETATE
  • TAE
  • TAE BUFFER
  • TAE BUFFER (10X)
  • TAE BUFFER (50X)
  • 2-AMINO-2-HYDROXYMETHYL-1,3-PROPANEDIOL ACETATE
  • 3-propanediol,2-amino-2-(hydroxymethyl)-acetate(salt)
CAS:
6850-28-8
MF:
C6H15NO5
MW:
181.19
EINECS:
229-939-6
Product Categories:
  • Buffer
  • Buffers
  • Protein Electrophoresis
  • SDS-PAGE
Mol File:
6850-28-8.mol
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Tris(hydroxymethyl)aminomethane acetate salt Chemical Properties

Melting point:
120-121 °C
Boiling point:
219-220 °C (9.7513 mmHg)
Density 
1.09 g/mL at 20 °C
storage temp. 
Inert atmosphere,Room Temperature
solubility 
H2O: 0.5 M at 20 °C, clear, colorless
form 
working solution
color 
White
Odor
Acetic odour
PH Range
6 - 7 at 91 g/l at 25 °C
PH
6.0-7.0 (25℃, 0.5M in H2O)
Water Solubility 
91 g/l at 20 °C - completely soluble
λmax
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.05
BRN 
3702918
InChIKey
PIEPQKCYPFFYMG-UHFFFAOYSA-N
CAS DataBase Reference
6850-28-8(CAS DataBase Reference)
EPA Substance Registry System
1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, acetate (salt) (6850-28-8)
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Safety Information

Hazard Codes 
Xn
Risk Statements 
36/37/38
Safety Statements 
26-36-24/25
WGK Germany 
3
3
HS Code 
29221990

MSDS

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Tris(hydroxymethyl)aminomethane acetate salt Usage And Synthesis

Chemical Properties

White Crystalline Powder

Uses

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

Uses

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Definition

ChEBI: An acetate salt resulting from the reaction of equimolar amounts of tris and acetic acid.

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