phosphoramide mustard
phosphoramide mustard Basic information
- Product Name:
- phosphoramide mustard
- Synonyms:
-
- phosphoramide mustard
- Phosphoramide Mustard D8
- CAS:
- 10159-53-2
- MF:
- C4H11Cl2N2O2P
- MW:
- 221.02
- Mol File:
- 10159-53-2.mol
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phosphoramide mustard Chemical Properties
- Melting point:
- 115 °C
- Boiling point:
- 363.5±52.0 °C(Predicted)
- Density
- 1.474±0.06 g/cm3(Predicted)
- pka
- 0.41±0.50(Predicted)
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phosphoramide mustard Usage And Synthesis
Definition
ChEBI: Phosphoramide mustard is a nitrogen mustard and a phosphorodiamide.
Biological Activity
Phosphoramide mustard is the active metabolite of cyclophosphamide (HY-17420) with antitumor activity. It induces DNA damage.
in vitro
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.
It (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells ( SIGCs).
Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.
It (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.
Cell Viability Assay
| Cell Line: | SIGCs |
| Concentration: | 0.5 μM, 1 μM, 3 μM, 6 μM |
| Incubation Time: | 48 hours |
| Result: | Reduced ce ll viability at concentrations of 3 μM and higher. |
RT-PCR
| Cell Line: | SIGCs |
| Concentration: | 3 μM, 6 μM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Increased DDR gene mRNA expression levels. |
Western Blot Analysis
| Cell Line: | SIGCs | < /tr>
| Concentration: | 3 μM, 6 μM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Generally increased DDR pro teins. |
target
DNA Alkylator
phosphoramide mustardSupplier
Chizhou Kailong Import and Export Trade Co., Ltd.
- Tel
- xg01_gj@163.com
Shenzhen Regent Biochemical Technology Co., Ltd.
- Tel
- 0755-0755-85201366 18938635012
- sales@regentsciences.com
AFINE CHEMICALS LIMITED
- Tel
- 0571-85134551 18958018566;
- info@afinechem.com
Guangzhou Yunmen Biotechnology Co., Ltd
- Tel
- 18902340307 18902340307
- 3356812514@qq.com
Guangdong Fangxin Biotechnology Co., Ltd.
- Tel
- 0751-2838688 13376594225
- 1276075424@qq.com
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phosphoramide mustard(10159-53-2)Related Product Information
- Cyclophosphamide monohydrate
- 4-HYDROPEROXY CYCLOPHOSPHAMIDE
- Mafosfamide
- Cyclophosphamide
- Defosfamide
- trofosfamide
- alcophosphamide
- carboxyphosphamide
- 4-hydroxycyclophosphamide
- phosphoramide mustard
- B 749
- 5,5-dimethylcyclophosphamide
- trans-5-Fluorocyclophosphamide
- Mafosfamide cyclohexylamine salt
- Carbamic acid, ((2-(bis(2-chloroethyl)amino)tetrahydro-2H-1,3,2-oxazap hosphorin-4-yl)oxy)-, phenylmethyl ester, P-oxide
- 2H-1,3,2-Oxazaphosphorine, tetrahydro-2-(bis(2-chloroethyl)amino)-5-ch loro-, 2-oxide, (Z)-
- 4-S-(Propionic acid)sulfidocyclophosphamide
- 4-(S-Ethanol)-sulfido-cyclophosphamide