NONYL ACRIDINE ORANGE
NONYL ACRIDINE ORANGE Basic information
- Product Name:
- NONYL ACRIDINE ORANGE
- Synonyms:
-
- NONYL ACRIDINE ORANGE
- NAO
- 3,6-BIS(DIMETHYLAMINO)-10-NONYLACRIDINIUM BROMIDE
- ACRIDINE ORANGE 10-NONYL BROMIDE
- 10-N-NONYL-3,6-BIS(DIMETHYLAMINO)ACRIDINIUM, BR
- 10-NONYLACRIDINE ORANGE BROMIDE
- 10-NONYLACRIDINE ORANGE BROMIDE, FOR FLU ORESCENCE
- 10-NONYLACRIDINE ORANGE BROMIDE FOR FLUORESCENCE 90+%
- CAS:
- 75168-11-5
- MF:
- C26H38BrN3
- MW:
- 472.5
- Product Categories:
-
- Acridine
- Mol File:
- 75168-11-5.mol
NONYL ACRIDINE ORANGE Chemical Properties
- Melting point:
- ≥250 °C(lit.)
- storage temp.
- 2-8°C
- solubility
- DMSO: soluble
- form
- powder
- color
- Orange-red
- Appearance
- Solid Powder
- ε(extinction coefficient)
- 76-88 at 491-497nm in methanol
- λmax
- 495 nm
- Biological Applications
- Detecting cardiolipin,mitochondrial membrane potential,risk of type 2 diabetes,cardiotoxicity,prostate cancer; apoptosis assay; drug screening assay; hematotoxicity assay; treating arthritic disorders; in photodynamic ther
NONYL ACRIDINE ORANGE Usage And Synthesis
Description
Acridine orange 10-nonyl is a metachromatic dye that is used to analyze mitochondria by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Uses
Nonyl acridine orange is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a usefull probe for following mitochondria during isolation and after cell fusion. This dye has been used to analyze mitochondria by flow cytometry,
in vivo
Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].
Excitation / Emission Maximum
490 nm/510 nm
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