Z-VAD-FMK
Z-VAD-FMK Basic information
- Product Name:
- Z-VAD-FMK
- Synonyms:
-
- Z-VADC(OME)-FMK
- methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-phenylmethoxycarbonylamino-butanoyl]amino]propanoyl]amino]-4-oxo-pentanoate
- N-BENZYLOXYCARBONYL-VAL-ALA-ASP FLUOROMETHYL KETONE
- Z-VAD-FLUOROMETHYLKETONE
- Z-VAD-FMK
- Z-VAD-FMK, METHYL ESTER
- Z-VAD(OME)-FLUOROMETHYLKETONE
- L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-
- CAS:
- 187389-52-2
- MF:
- C22H30FN3O7
- MW:
- 467.49
- Product Categories:
-
- Inhibitors
- Caspases/Apoptosis
- Caspase/Related Products
- Apoptosis
- peptides
- API
- Intermediates
- Mol File:
- Mol File
Z-VAD-FMK Chemical Properties
- Melting point:
- >190°C (dec.)
- Boiling point:
- 732.4±60.0 °C(Predicted)
- Density
- 1.214±0.06 g/cm3 (20 ºC 760 Torr)
- Flash point:
- 396.7℃
- storage temp.
- -20°C
- solubility
- DMSO: 20 mg/mL
- form
- powder
- pka
- 11.05±0.46(Predicted)
- color
- white
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
- InChIKey
- MIFGOLAMNLSLGH-QOKNQOGYSA-N
- SMILES
- C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF
- CAS DataBase Reference
- 187389-52-2
Safety Information
- Safety Statements
- 22-24/25
- WGK Germany
- 3
- HS Code
- 29145090
MSDS
- Language:English Provider:SigmaAldrich
Z-VAD-FMK Usage And Synthesis
Description
Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.
Uses
Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]
Uses
Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.
Definition
ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.
General Description
A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.
Biological Activity
Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .
Biochem/physiol Actions
Cell permeable: yes
Enzyme inhibitor
This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.
storage
-20°C
References
1) Slee et al. (1996), Benzyloxycarbonyl-Val-Ala-ASP (OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32; Biochem. J., 315 21 2) Kunstle et al. (1997), ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha; Immunol. Lett., 55 5 3) Garcia-Calvo et al. (1998), Inhibition of human caspases by peptide based and macromolecular inhibitors; J. Biol. Chem., 273 32608
Z-VAD-FMKSupplier
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- 17740884366
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- 18914398991
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- 0571-87213919
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- 025-58849295 18951903616;
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Z-VAD-FMK(187389-52-2)Related Product Information
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