Hoechst 33342 analog Chemical Properties

Boiling point:

841.2±75.0 °C(Predicted)

Density 

1.303±0.06 g/cm3(Predicted)

solubility 

25℃: DMSO or water Protect from light

form 

Powder

pka

11.02±0.10(Predicted)

color 

Off-white to brown

Safety Information

Toxicity

mnt-ham-ovr 5 mmol/L MUREAV 448,35,2000

Hoechst 33342 analog Usage And Synthesis

Uses

Hoechst 33342 analog is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

Safety Profile

Mutation data reported. Whenheated to decomposition it emits toxic vapors of NOx andCl-.

References

[1] Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557. DOI:10.1101/pdb.prot5557