Piperazine-N,N'-bis-(2-ethanesulphonic acid) dipotassium salt Chemical Properties

storage temp. 

Store at RT.

solubility 

H₂O: 0.5 g/mL, clear, colorless

form 

crystalline powder

PH

6.1-7.5

pka

6.8(at 25℃)

PH Range

6.1 - 7.5

Water Solubility 

water: 0.5g/mL, clear, colorless

CAS DataBase Reference

108321-27-3(CAS DataBase Reference)

EPA Substance Registry System

1,4-Piperazinediethanesulfonic acid, dipotassium salt (108321-27-3)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-37/39

WGK Germany 

3

F 

10

HS Code 

29335995

MSDS

• Language:English Provider:SigmaAldrich

Piperazine-N,N'-bis-(2-ethanesulphonic acid) dipotassium salt Usage And Synthesis

Chemical Properties

White crystalline powder

Uses

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

General Description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

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