Solvent Red 43 Chemical Properties

Melting point:

300 °C

Boiling point:

640.3±55.0 °C(Predicted)

Density 

1.02 g/mL at 20 °C

refractive index 

1.5000 (estimate)

storage temp. 

Inert atmosphere,Room Temperature

solubility 

H₂O: 0.1 g/mL, dark red

form 

Liquid

Colour Index 

45380:2

pka

6.97±0.20(Predicted)

color 

White to Light yellow to Red

λmax

524 nm

Stability:

Stable. Incompatible with strong oxidizing agents.

InChIKey

DBZJJPROPLPMSN-UHFFFAOYSA-N

CAS DataBase Reference

15086-94-9

IARC

3 (Vol. 15, Sup 7) 1987

EPA Substance Registry System

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',4',5',7'-tetrabromo-3',6'-dihydroxy- (15086-94-9)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36

Safety Statements 

26-24/25

RIDADR 

UN 1170 3/PG 2

WGK Germany 

3

RTECS 

LM5850000

HS Code 

32041919

MSDS

• Language:English Provider:3,6-Dihydroxy-2,4,5,7-tetrabromospiro(xanthene-9,3'-phthalide)
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich

Solvent Red 43 Usage And Synthesis

Chemical Properties

orange-brown powder

Uses

Eosin Acid C.I. No. 45380 (CAS# 15086-94-9) is a photo-senseitive organic catalyst and has been used in the photocatalytic, diastereoselective additions of 1-sulfoximidoyl-1,2-benziodoxoles to styrenes, and in the stereoselective synthesis of 2-deoxyglycosides from glycals. Dyes and metabolites.

Definition

ChEBI: Bromoeosin is an organobromine compound. It is functionally related to a fluorescein (lactone form).

Preparation

Fluorescein (C.I. Acid Yellow 73, C.I. 45350) in water or ethanol solution bromide into four bromine derivatives, And made into a free acid form..

Biological Activity

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Synthesis

The general procedure for the synthesis of 2',4',5',7'-tetrabromo-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthene]-3-one from 3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthene] is as follows: fluorescein in the form of the lactone (1 eq.), NaBr (16 eq.), and Oxone? ( 6 equiv, 2KHSO5-KHSO4-K2SO4) were mixed and ground in an agate mortar until uniformly fine. The resulting solid mixture was reacted in an oven at 200 °C for 12 h. Upon completion of the reaction, the target product tetrabromofluorescein (4Br-F) was obtained in near 100% yield in the commercial disodium salt form known as eosin Y. The reaction mixture was diluted with water and the product was extracted with ethyl acetate. The product was characterized by mass spectrometry (ESI-MS) and spectroscopic techniques (1H-NMR and 13C-NMR). The specific data were as follows: ESI-MS (+) of 4Br-F: m/z 670.4 [M + H]+; 1H NMR (CH3OD): δ 7.04 ppm (s, 1H and 8H), 7.23 (d, J = 8.0 Hz, 3H), 7.72 (ddd, J = 8.0, 8.0, 1.2 Hz, 5H), 7.79 (ddd, J = 8.0, 8.0, 1.2 Hz, 4H), 8.09 ppm (d, J = 8.0 Hz, 6H).

Properties and Applications

brilliant yellow red. Soluble in water and ethanol for blue light red, with yellow green fluorescence. In concentrated sulfuric acid to yellow, dilution after yellow red precipitation.

References

[1] Patent: US2008/61289, 2008, A1. Location in patent: Page/Page column 6-7
[2] Fortschr. Teerfarbenfabr. Verw. Industriezweige, vol. 5, p. 216
[3] Journal of the American Chemical Society, 1927, vol. 49, p. 1278
[4] Justus Liebigs Annalen der Chemie, 1876, vol. 183, p. 31
[5] Justus Liebigs Annalen der Chemie, 1910, vol. 372, p. 109

Solvent Red 43(15086-94-9)Related Product Information

• Fluorescein isothiocyanate
• Fluorescein Sodium
• EC 1.14.14.3
• Phthalide
• Hydroxy silicone oil
• Furazolidone
• FURFURYL ALCOHOL RESIN
• Styrene-acrylic latex
• Xanthene
• Tetrahydrofuran
• Nitrofurantoin
• ROSE BENGAL
• Fluorescein
• EOSIN
• Solvent Red 1
• Solvent Red 8
• Solvent Red 49
• Solvent Red 30