PHOSPHORYLCHOLINE CHLORIDE CALCIUM SAL& Chemical Properties

storage temp. 

-20°C

solubility 

Water (Slightly)

Water Solubility 

Soluble in water

form 

powder to crystal

color 

White to Almost white

biological source

synthetic (organic)

Merck 

14,7362

BRN 

4835230

Stability:

Stable, but light sensitive and may also be heat sensitive. Store at -20℃.

InChI

InChI=1S/Ca.2Cl2HO2P.4H2O/c;2*1-5(2,3)4;;;;/h;2*(H,3,4);4*1H2/q+2;;;;;;/p-2

InChIKey

WOCFGZLMOWLHFB-UHFFFAOYSA-L

SMILES

[Ca+2].ClP(Cl)([O-])=O.ClP(Cl)([O-])=O.[H]O[H].[H]O[H].[H]O[H].[H]O[H]

CAS DataBase Reference

72556-74-2

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

HS Code 

2923.90.0100

Storage Class

11 - Combustible Solids

Hazard Classifications

Eye Irrit. 2
Skin Irrit. 2
STOT SE 3

PHOSPHORYLCHOLINE CHLORIDE CALCIUM SAL& Usage And Synthesis

Description

Phosphocholine chloride calcium salt tetrahydrate is a phospholipid and also an essential component of cell membranes. It is involved in many cellular processes such as cell growth, differentiation, membrane transport and signal transduction, and can be used to study the effects of phospholipids on cell growth and differentiation. It can also be used as an intermediate raw material for synthetic enzymatic hydrolysis of lignin (EHL)-grafted phosphobetaine (EHLPB).

Chemical Properties

white powder

Uses

Phosphocholine Chloride is a specific promoter of heparin and serum β-lipoprotein interaction. Phosphocholine Chloride protected the membranes of platelets, erythrocytes, and lysosomes against the labilizing action of various known membrane labilizers.

Synthesis

1. 1032 g of orthophosphoric acid was added to a 2L four-neck flask and heated to 160-165°C. 2. 2. a mixed clarified solution consisting of 1032 g of orthophosphoric acid and 852 g of choline chloride was added slowly and dropwise, maintaining the temperature at 160°C for 2 hours, during which time it was ensured that no water vapor was generated and about 520 g of distillate was collected. 3. Transfer the reaction solution to a 50L glass reactor, cool to 30°C, add 15kg of pure water and stir until the solution becomes colorless and transparent, at which time the pH should be 1-2. 4. Add 520g of calcium chloride and stir at room temperature until completely dissolved. 5. 5. Slowly add 900g of calcium carbonate, observe a large number of foam generation and the liquid becomes white milky, stirring at room temperature for 30 minutes. 6. Add 540g of calcium hydroxide, continue to stir at room temperature for 30 minutes, adjust and maintain the pH to 8.0, the liquid is still white milky. 7. Add 2kg of purified water, make sure the pH is stabilized at 8.0, then centrifuge and filter, and wash the filter cake with purified water. 8. Calcium hydroxide was added to the filter cake, the pH was adjusted to 9-13, centrifugation was performed again, and 2.3 kg of calcium phosphate solid was obtained after drying. 9. The filtrate was filtered through a 0.2 μm PP microfiltration membrane to obtain 21 kg of colorless transparent liquid. 10. The liquid was concentrated to 4L using an experimental ultrafiltration membrane at 1.5MPa and 20-30°C. The liquid was then filtered through a 0.5μm PP microfiltration membrane. 11. Add 1.5L of 95% ethanol, stir for 30 minutes and then crystallize at 0-5°C for 3 hours and vacuum filter. 12. Wash the filter cake with 2150 ml of 75% ethanol/pure water (1:1 v/v) and stir the mixture at room temperature. 13. Add 10L of 95% ethanol, the solution gradually changed from turbid to milky white, left to drain. 14. Dry the wet product in vacuum at 60 ℃ for 3 hours to get 1052g N,N,N-trimethyl-2-(phosphonooxy)ethanaminium chloride calcium salt tetrahydrate dry product. 15. Product analysis: Chlorine content 10.7%, phosphorus content 9.5%, calcium content 12.4%, titer content 98.8%. Infrared spectrum conforms to the reference standard.

IC 50

Human Endogenous Metabolite; Microbial Metabolite

References

[1] Patent: CN103936785, 2016, B. Location in patent: Paragraph 0027-0032

PHOSPHORYLCHOLINE CHLORIDE CALCIUM SAL&(72556-74-2)Related Product Information

• Lecithin
• Choline glycerophosphate
• Phosphoric acid
• Calcium chloride
• Calcium chloride hexahydrate
• phosphorylcholine
• CHOLINE BROMIDE
• Choline chloride