RED BLOOD CELL LYSING BUFFER HYBRI-MAX Chemical Properties

form 

liquid

Safety Information

WGK Germany 

3

RED BLOOD CELL LYSING BUFFER HYBRI-MAX Usage And Synthesis

Uses

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

RED BLOOD CELL LYSING BUFFER HYBRI-MAX()Related Product Information

• Ordinary RIPA tissue/cell lysate
• ACK red blood cell lysate
• Western and IP Cell lysis Buffer
• Native lysis Buffer
• RIPA lysate (strong, no inhibitor)
• RIPA Lysate (middle)
• SDS lysate
• NP-40 Lysis Buffer
• Erythrocyte lysate type B (for flow cytometry)
• SDS cell lysate
• RED BLOOD CELL LYSIS BUFFER Erythrocyte Lysate
• LYSOZYME/lysozyme (>20KU/MG)
• Single cell lysate (DNA)
• Denature Lysis Buffer
• Complete Cell Lysis solution (CCLTM)
• Tissue Total Protein Lysis Buffer
• Bacterial Lysis Buffer
• Bacterial Activity-Keeping Lysis Buffer