NAPHTHOL AS-BI PHOSPHATE Chemical Properties

Density 

1.679±0.06 g/cm3(Predicted)

Flash point:

37 °C

storage temp. 

2-8°C

solubility 

methanol: soluble50mg/mL

pka

0.73±0.30(Predicted)

form 

Crystalline Powder

color 

Light yellow

Appearance

Solid Powder

BRN 

6668526

InChI

InChI=1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)

InChIKey

HUXIAXQSTATULQ-UHFFFAOYSA-N

SMILES

C1=C2C(C=CC(Br)=C2)=CC(OP(O)(O)=O)=C1C(NC1=CC=CC=C1OC)=O

CAS DataBase Reference

1919-91-1

EPA Substance Registry System

2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)- (1919-91-1)

Safety Information

Hazard Codes 

T,Xi,C,F

Risk Statements 

61-20/21-36/37/38-39/23/24/25-20/21/22-10-34-11-36

Safety Statements 

7-16-36/37-45-36-26-36/37/39-23-53

RIDADR 

UN 2265 3/PG 3

WGK Germany 

3

F 

8-10-21

HS Code 

29089990

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

NAPHTHOL AS-BI PHOSPHATE Usage And Synthesis

Description

Naphthol AS-BI-phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS-BI-phosphate is cleaved to release the fluorescent moiety naphthol AS-BI (N-ASBI), which can be used to quantify the activity of acid and alkaline phosphatases. Acid and alkaline phosphatases are commonly used for diagnosis of diseases.

Chemical Properties

Light yellow crystalline powder

Uses

Naphthol AS-BI phosphate is suitable to use:

• in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
• as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
• to perform alkaline phosphatase reaction

', 'Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Biological Activity

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

NAPHTHOL AS-BI PHOSPHATE(1919-91-1)Related Product Information

• BENZANILIDE
• o-Anisidine
• NAPHTHOL AS-BI PHOSPHATE SODIUM*SIGMAULT RA,NAPHTHOL AS-BI PHOSPHATE SODIUM,naphthol as-bi phosphate disodium salt,NAPHTHOL AS-BI PHOSPHATE SODIUM SALT
• DIMETHYLAMINE HYDROBROMIDE
• N-(2,3-Dihydro-2-oxo-1H-benzimidazol-5-yl)-3-hydroxy-2-naphthalenecarboxamide
• NAPHTHOL AS-OL PHOSPHATE
• 2-METHOXY-N-METHYANILINE
• Methyl Phosphate (Mono- and Di- Ester mixture)
• BETA-NAPHTHYL PHOSPHATE
• 2-Acetamidophenol
• 2-Bromonaphthalene
• Salicylanilide
• TRI-AMMONIUM PHOSPHATE TRIHYDRATE
• 2-METHYLAMINOPHENOL
• N-Methylbenzamide
• ETHYLAMINE HYDROBROMIDE
• 2-Hydroxy-3-naphtoamide
• PHENYLPHOSPHORIC ACID