Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Chemical Properties

Melting point:

>181oC (dec.)

storage temp. 

2-8°C

solubility 

H₂O: 50 mg/mL, very slightly hazy, green

form 

tablet

color 

Pale Green to Light Green

PH

pH(50g/l, 25℃) : 5.0～6.0

Water Solubility 

Dissolve in water at 50mg/ml. Solution may be slightly hazy.

Sensitive 

Light Sensitive

BRN 

7329461

Stability:

Hygroscopic, Light Sensitive

CAS DataBase Reference

30931-67-0(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

RTECS 

DL7002000

F 

8-10-23

HS Code 

29342080

MSDS

• Language:English Provider:SigmaAldrich

Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Usage And Synthesis

Chemical Properties

Light green powder

Uses

Chromogenic substrate for peroxidase assays.2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt is widely utilized as a peroxidase substrate suitable for use in enzyme-linked immunosorbent assay (ELISA) procedures. It plays an important role in the food industry to measure the antioxidant capacities of foods. It is used to observe the reaction kinetics of specific enzymes and involved in glucose estimating reagent in blood serum.

Uses

• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).?
• ABTS is also used to measure glucose concentrations in different solutions including blood serum.
• The food industry uses ABTS to measure the antioxidant capacities within different foods.
• Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
• It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Uses

Diammonium-2,2’Azino-bis(3-ethylbenzothiazoline-6-sulfonate) has been shown to inhibit catalase, a mammalian antioxidant enzyme which degrades hydrogen peroxide into water and oxygen species.

General Description

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate:

• ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example).
• ABTS together with laccase or bilirubin oxidase.

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).??? ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.

Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)(30931-67-0)Related Product Information

• Sodium isethionate
• 2,2'-Dithiobis(benzothiazole)
• Polyquaternium-10
• Benzothiazole
• 2-Mercaptobenzothiazole
• Behenyl Trimethyl Ammonium Chloride
• Ascoric Acid
• 2-IMINO-3-METHYLBENZOTHIAZOLE
• MBTH INDICATOR
• 2-(METHYLTHIO)ANILINE
• N-METHYLANILINE-P-SULFONIC ACID
• 2-Methyl-2-thiopseudourea sulfate
• 1,3-BENZENEDITHIOL
• Ethylbenzene
• 4-(dimethylamino)thiophenol
• benzothiazoline
• N-METHYL-THIOCARBAMIDINE METHYL ESTER
• CITRATE BUFFER FOR ABTS
• Abts Diammonium Salt