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Basic Violet 2

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Basic Violet 2 Basic information

Product Name:
Basic Violet 2
Synonyms:
  • 4-[(4-Amino-3-methylphenyl)-(4-imino-3-methyl-1-cyclohexa-2,5-dienylidene)methyl]-2-methylaniline hydrochloride
  • Benzenamine, 4-((4-amino-3-methylphenyl)(4-imino-3-methyl-2,5-cyclohexadien-1-ylidene)methyl)- 2-methyl-, monohydrochloride
  • C.I.Basic Violet 24
  • Isorubin
  • New Fuchsin [for BiocheMical Research]
  • NEW FUCHSIN (C.I. 42520) FOR MICROSCOPY
  • New fuchsin (C.I. 42520) for microscopy Certistain
  • Basic Violet 2, FuchsinN, Magenta(R) III
CAS:
3248-91-7
MF:
C22H24ClN3
MW:
365.91
EINECS:
221-831-7
Mol File:
3248-91-7.mol
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Basic Violet 2 Chemical Properties

Boiling point:
300℃[at 101 325 Pa]
Density 
0.692[at 20℃]
vapor pressure 
0Pa at 25℃
storage temp. 
Store at +5°C to +30°C.
solubility 
10g/l
Colour Index 
42520
form 
Crystals or Crystalline Powder
color 
Dark green
PH
3-4 (10g/l, H2O, 20℃)
Water Solubility 
20g/L
BRN 
8740867
Stability:
Stable. Incompatible with strong oxidizing agents.
LogP
1.537
CAS DataBase Reference
3248-91-7(CAS DataBase Reference)
EPA Substance Registry System
Benzenamine, 4,4'-[(4-imino-3-methyl-2,5-cyclohexadien-1-ylidene)methylene]bis[2-methyl-, hydrochloride (1:1) (3248-91-7)
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Safety Information

Hazard Codes 
Xn,Xi,N,T
Risk Statements 
36/37/38-40-36-50/53
Safety Statements 
26-36/37-24/25-61-60
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3
RTECS 
CX9825000
HS Code 
32041300

MSDS

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Basic Violet 2 Usage And Synthesis

Chemical Properties

solid

Uses

Textiles and leather industries, red dye, pharmaceutical.

Definition

ChEBI: A hydrochloride that is the monohydrochloride of 4-[(4-aminophenyl)(4-iminocyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline. One of the minor constituents of Basic fuchsin, together with pararosanilin, rosanilin and magenta II.

Preparation

Commonly known as the New Magenta. 4 – (4-Amino-3-methylbenzyl)-2-methylbenzenamine with O-Methylaniline its hydrochloride salt is heated in the presence of an oxidizing agent.

General Description

The dry dye New fuchsin (C.I. 42520) for microscopy Certistain?, is used for the preparation of a staining solution that can be used for staining of histological tissue sections or other samples of human origin according to Ziehl-Neelsen, Gram staining as well as in combination with Schiff′s reagent. As a Certistain? dye it is chemically quality-certified according to highest standards and strict specifications. New fuchsin belongs to the group of triaminotriphenylmethane dyes.
The dye is used in Ziehl-Neelsen and Gram staining as well as for methods with Schiff′s reagent.
The cell wall of acid-fast bacteria (AFB) has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided.
Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
Pretreatment of the specimens with Sputofluol? dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol? also has a disinfectant effect, with the result that any microorganisms that are present are killed off. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Hazard

Possible carcinogen.

Flammability and Explosibility

Not classified

Properties and Applications

dark red light purple. Soluble in water and ethanol is red. The strong sulfuric acid for yellow until golden brown, diluted into light yellow. Dyes to join sodium hydroxide solution appears orange brown precipitation.

Standard Light Fastness Persperation Fastness Ironing Fastness Soaping
Fading Stain Fading Stain Fading Stain
ISO 3 4 4-5 4-5 5
AATCC 3

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