D-BIOTIN P-NITROPHENYL ESTER Chemical Properties

Melting point:

163-165 °C(lit.)

Boiling point:

642.9±50.0 °C(Predicted)

Density 

1.326±0.06 g/cm3(Predicted)

storage temp. 

Sealed in dry,Room Temperature

solubility 

methanol: soluble25mg/mL, clear, colorless to greenish-yellow

pka

13.89±0.40(Predicted)

form 

Solid

color 

White to Off-White

biological source

mouse

optical activity

[α]25/D +51°, c = 2 in DMF: AcOH (99:1)

BRN 

5795805

InChIKey

YUDNXDTXQPYKCA-OWYJLGKBSA-N

CAS DataBase Reference

33755-53-2

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

HS Code 

29349990

MSDS

• Language:English Provider:SigmaAldrich

D-BIOTIN P-NITROPHENYL ESTER Usage And Synthesis

Chemical Properties

White to off-white powder

Uses

(+)-Biotin 4-nitrophenyl ester may be used in the preparation of biotin tagged ARC (adenosine-oligoarginine conjugate) derivative during cellular protein uptake studies. It may also be used to prepare biotinylated quinazoline derivatives, which shows inhibitory activities against VEGFR-2 (vascular endothelial growth factor receptor-2) tyrosine kinase. (+)-Biotin 4-nitrophenyl ester undergoes hydrolysis in the presence of Na[¹⁷O]H to form [¹⁷O₂]biotin, which can be used in solid-state ¹⁷O NMR spectroscopic experiments to analyze large protein-ligand complexes.

Uses

For research use only. Not for use in diagnostic procedures.
Brain natriuretic peptide EIA Kit has been used to measure brain natriuretic peptide (BNP) secreted in human neonatal ventricular cardiomyocyte culture.

General Description

The BNP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting BNP peptide based on the principle of Competitive Enzyme Immunoassay.

reaction suitability

reaction type: Biotinylations

Biochem/physiol Actions

Brain natriuretic peptide (BNP) expressed in mammalian brain and atrial natriuretic peptide (ANP) facilitates various physiological functions including regulation of blood pressure and cardiac remodeling. BNP acts as a potential biomarker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular (LV) dysfunction. Elevated expression of BNP is observed in acute myocardial infarction (AMI) patients. BNP is also implicated in several biological functions such as diuresis, natriureis, hypotensive action and inhibition of aldosterone secretion.

Purification Methods

The ester has been recrystallised by dissolving 2g in 95% EtOH (30mL), heated to dissolve, then cooled in an ice-water bath. The crystals are collected, washed with ice-cold 95% EtOH (5mL) and dried over P2O5. The RF on silica plates (CHCl3/MeOH 19:1) is 0.19 [Bodanszky & Fagan J Am Chem Soc 99 235 1977].

Description

B-type Natriuretic Peptide Recombinant Human produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 32 amino acids and having a molecular mass of 3,500 Dalton. NPPB is purified by proprietary chromatographic techniques. 

Source

Escherichia Coli

Background

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body's salt and water balance. Improves heart function.

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