CY3 NHS ESTER Chemical Properties

storage temp. 

under inert gas (nitrogen or Argon) at 2-8°C

solubility 

DMSO: soluble

form 

A crystalline solid

color 

Brown to black

Appearance

dark red crystals

InChIKey

SOVMUUCAZOFIQP-UHFFFAOYSA-N

SMILES

C(N1C(C(C)(C)C2=CC(S(O)(=O)=O)=CC=C12)=CC=CC1C(C2=CC(S([O-])(=O)=O)=CC=C2[N+]=1CC)(C)C)CCCCC(=O)ON1C(CCC1=O)=O

Safety Information

HS Code 

29349990

CY3 NHS ESTER Usage And Synthesis

Description

Water soluble, amino-reactive sulfo-Cyanine3 NHS ester that efficiently labels proteins and peptides in purely aqueous solution without needing organic co-solvent. Ideal for proteins with low solubility and proteins prone to denaturation.

This is the sulfonated, hydrophilic, and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.

This product is an analog of Cy3® NHS ester. sulfo-Cyanine3 NHS ester replaces Cy3® and DyLight 549 for all applications.

Uses

Fluorescent dyes are typically used for imaging applications. Cy3 NHS Ester is a fluorescent dye suitable for labeling proteins and nucleic acids. It exhibits excitation and emission at 555 nm and 570 nm, respectively. Cy3 NHS Ester can be used to label functional nucleic acids to detect tertiary structure folding such as guanine quadruplex.

Chemical Reactivity

Primary amine

Abs/Em Maxima

548/563 nm

Excitation / Emission Maximum

555 nm/570 nm

Fluoresene quantum yield

0.31

Extinction Coefficient

150000

CF260

0.03

CF280

0.06

Spectrally Similar Dyes

Cy3, DyLight® 555, Alexa Fluor® 555

CY3 NHS ESTER(146368-16-3)Related Product Information

• Cy5
• Cy3 DBCO
• Cyanine3 alkyne
• Cyanine3B carboxylic acid
• Cyanine3 DBCO
• Cy3 NHS Ester
• Cy7
• Cy3
• CY5 NHS ESTER
• Calcein tetraethyl ester