Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin Chemical Properties

solubility 

good in water, DMF, DMSO

Appearance

dark blue solid

Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin Usage And Synthesis

Description

sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.

Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.

sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.

The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.

Abs/Em Maxima

646/662 nm

Fluoresene quantum yield

0.28

Extinction Coefficient

271000

CF260

0.04

CF280

0.04

Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5-PEG3-Biotin()Related Product Information

• Sulfo-Cyanine5.5 maleimide
• Cyanine5.5 hydrazide
• Sulfo-Cyanine5.5 alkyne
• Sulfo-Cyanine5.5 carboxylic acid
• sulfo-Cyanine5.5 tetrazine
• Sulfo-CY5.5 DBCO
• Sulfo-Cyanine5.5 azide
• Sulfo-Cyanine5.5 amine
• Sulfo-Cyanine5 bis-NHS ester