GRANZYME B Chemical Properties

storage temp. 

-70°C

form 

buffered aqueous solution

Safety Information

Risk Statements 

42/43

Safety Statements 

26-36

WGK Germany 

-

MSDS

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GRANZYME B Usage And Synthesis

General Description

Granzyme B, a 247 amino acid polypeptide, contains a leader sequence, which is cleaved by a signal peptidase and a two amino acid prodomain, which is cleaved by the lysosomal cysteine protease DPPI. The recombinant granzyme B is expressed in Pichia pastoris as the mature form and appears on SDS-PAGE as a triplet (~34, 32, and 30 kDa) due to three different glycosylations.

Biochem/physiol Actions

Cytotoxic granules secreted by natural killer (NK) cells and cytotoxic T Lymphocytes cells are part of the mechanism used for protecting the organism from virus infected and tumor cells. Granzyme B, a serine protease, is the most prominent granzyme in a family of 11 found in the cytotoxic granules. The granzymes enter the target cell with the assistance of perforin, a critical molecule of the cytotoxic granules. In the target cell, the granzymes act on specific substrates involved with the cell death via apoptosis.Granzyme B is a neutral serine protease that cleaves aspartic acid residues, inducing cell death by various pathways. It can cleave and activate most of the caspases in vitro and in vivo resulting in a massive amplification of the caspase dependent apoptotic pathway. In addition, granzyme B cleaves directly downstream caspase substrates as PARP DNA-PK2 and DFF45/ICAD leading to cell death. This pathway bypasses inhibition of apoptosis by viral caspase inhibitors found in virus infected cells. It has been shown that granzyme B is capable of inducing cytochrome C release from the mitochondria in a caspase independent way.

GRANZYME B(143180-74-9)Related Product Information

• METHYL ISOCYANOACETATE
• 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
• Tosylmethyl isocyanide
• DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
• TERT-BUTYL ISOCYANIDE
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
• SALCOMINE
• 2,4-PENTANEDIONE, SILVER DERIVATIVE
• N-BUTYLISOCYANIDE
• Ethyl isocyanoacetate
• Cupric acetylacetonate
• Ferric acetylacetonate
• COBALT(II) ACETYLACETONATE
• Tris(2,4-pentanedionato)chroMiuM(III)
• PHENYLSELENOL
• Benzyl isocyanide
• Aluminum acetylacetonate