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2'-OMe-ibu-G Phosphoramidite

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2'-OMe-ibu-G Phosphoramidite Basic information

Product Name:
2'-OMe-ibu-G Phosphoramidite
Synonyms:
  • 2’-O-Methy-rG(N-ibu)Phosphoramidite
  • N-blocked-5'-O-DMT-2'-O-Me CED guanosine phosphoramidite
  • 5'-O-(4,4-Dimethoxytrityl)-2'-O-methyl-N-isobutyrylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
  • 5'-O-DMT-2'-O-Me-rG(N-iBu)-3'-CEDPA
  • 2'-OMe-ibu-G Phosphoramidite
  • 5'-O-DMT-2'-O-Me-G(ibu) phosporamidite
  • DMT-2′O-Methyl-rG(ib) Phosphoramidite
  • ibu-2'-OMe-G Phosphoramidite
CAS:
150780-67-9
MF:
C45H56N7O9P
MW:
869.94
EINECS:
2017-001-1
Product Categories:
  • Amidite
Mol File:
150780-67-9.mol
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2'-OMe-ibu-G Phosphoramidite Chemical Properties

Melting point:
138 - 140°C
storage temp. 
Inert atmosphere,2-8°C
solubility 
Acetonitrile (Slightly), Chloroform (Slightly), Methanol (Slightly, Sonicated)
form 
powder
pka
9.16±0.20(Predicted)
color 
white to off-white
InChI
InChI=1/C45H56N7O9P/c1-28(2)41(53)49-44-48-40-37(42(54)50-44)47-27-51(40)43-39(57-9)38(61-62(59-25-13-24-46)52(29(3)4)30(5)6)36(60-43)26-58-45(31-14-11-10-12-15-31,32-16-20-34(55-7)21-17-32)33-18-22-35(56-8)23-19-33/h10-12,14-23,27-30,36,38-39,43H,13,25-26H2,1-9H3,(H2,48,49,50,53,54)/t36-,38-,39-,43-,62?/s3
InChIKey
IRRDHRZUOZNWDJ-MLLDKZSOSA-N
SMILES
O(C(C1=CC=C(OC)C=C1)(C1=CC=C(OC)C=C1)C1=CC=CC=C1)C[C@H]1O[C@@H](N2C3=C(C(NC(=N3)NC(=O)C(C)C)=O)N=C2)[C@H](OC)[C@@H]1OP(N(C(C)C)C(C)C)OCCC#N |&1:25,27,44,47,r|
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Safety Information

WGK Germany 
3
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2'-OMe-ibu-G Phosphoramidite Usage And Synthesis

Uses

2''-OMe-ibu-G Phosphoramidite (CAS# 150780-67-9) is used in the synthesis of native-like crosslinked duplex RNA via interstrand crosslinking reaction between vinylpurine on one strand and target cytosine or uracil on the opposite strand.

Definition

2'-OMe-G(iBu)-3'-phosphoramidite is a chemically unstable and heat-sensitive nucleoside phosphoramidite that serves as a foundational element in oligonucleotide synthesis. 2'-OMe-RNA CE (β-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

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