Giemsa stain Chemical Properties

Melting point:

300 °C(lit.)

Boiling point:

>65°C/1013hPa

bulk density

520kg/m3

Density 

0.99

Flash point:

15 °C

storage temp. 

room temp

solubility 

ethanol: 1 mg/mL

form 

powder

color 

Greenish-gold

PH

pH (25℃) : 6.0～8.0

explosive limit

5.5-44% (v/v) Methanol)

Water Solubility 

Insoluble in water (<0.1%), methanol (5.8 mg/L), and ethanol.

λmax

645-655 nm

CAS DataBase Reference

51811-82-6(CAS DataBase Reference)

EPA Substance Registry System

Giemsa's stain (51811-82-6)

Absorption

≥0.6 at 647-653nm in methanol at 0.005g/L

Safety Information

Hazard Codes 

Xi,T,F,Xn

Risk Statements 

41-39/23/24/25-23/24/25-11-63-62-46-36/38-21-52/53-22

Safety Statements 

7-16-36/37-45-39-26-53-25-61

RIDADR 

UN 1230 3/PG 2

WGK Germany 

3

F 

12

Autoignition Temperature

455°C

TSCA 

Yes

HS Code 

32129000

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Giemsa stain Usage And Synthesis

Chemical Properties

DARK GREEN TO GREENISH-BLACK FINE CRYST. POWDER

Uses

Giemsa Stain is used as a laboratory reagent and stain for microscopy. It is also used for the histopathologic detection of malaria and other parasites such as spirochete and protozoan. The stain is also been incorporated in differential staining between bacterial and human cells. The compound has been used in combination with May-Grunwald or Wright Stain (sc-203763) as a histological hematology stain.

Uses

Giemsa stain has been used for the staining of cells and blood films.

Definition

ChEBI: Azure A is an organic chloride salt having 3-amino-7-(dimethylamino)phenothiazin-5-ium as the counterion. It is used in making azure eosin stains for blood smear staining. It has a role as a histological dye and a fluorochrome. It contains a 3-amino-7-(dimethylamino)phenothiazin-5-ium.

General Description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

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