DISUCCINIMIDYL SUBERATE Chemical Properties

Melting point:

169-170°C

Boiling point:

513.5±60.0 °C(Predicted)

Density 

1.295 g/cm3

storage temp. 

2-8°C

solubility 

chloroform: 50 mg/mL

form 

powder

color 

White to Almost white

Appearance

White solid

Water Solubility 

Insoluble in water. Soluble in chloroform: 50 mg/mL.

InChI

InChI=1S/C16H20N2O8/c19-11-7-8-12(20)17(11)25-15(23)5-3-1-2-4-6-16(24)26-18-13(21)9-10-14(18)22/h1-10H2

InChIKey

ZWIBGKZDAWNIFC-UHFFFAOYSA-N

SMILES

C(ON1C(=O)CCC1=O)(=O)CCCCCCC(ON1C(=O)CCC1=O)=O

CAS DataBase Reference

68528-80-3

Safety Information

Safety Statements 

24/25

WGK Germany 

3

HS Code 

29171900

MSDS

• Language:English Provider:SigmaAldrich

DISUCCINIMIDYL SUBERATE Usage And Synthesis

Description

DSS Crosslinker is a membrane permeable, noncleavable crosslinker. The NHS ester groups are reactive toward primary amines.

Uses

Disuccinimidyl suberate (DSS) is a bifunctional cross-linking reagent which contains two N-hydroxysuccinimide esters that are reactive toward primary amines. DSS is membrane-permeable and is commonly used to cross-link proteins in intact cells.

Uses

Disuccinimidyl suberate is a homobifunctional amine-reactive crosslinker. It is usually coupled to molecules containing primary amines via amide bonds buffered at pH 7.5 (6.5-8.5). It incorporates an 8-atom spacer arm.

Uses

Suberic acid bis(N-hydroxysuccinimide ester) has been:

• supplemented in pierce lysis buffer used for immunoprecipitation
• used to conjugate the oligonucleotide standards to gelatin
• used in cross-linking of ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) oligomers

General Description

Suberic acid bis(N-hydroxysuccinimide ester) is a homobifunctional cross-linking reagent with amine reactivity. Typically coupled to molecules containing primary amine by amide bond buffered at pH 7.5 (6.5-8.5). Suberic acid bis(N-hydroxysuccinimide ester) incorporates an 8-atom linker.

reaction suitability

reagent type: cross-linking reagent

IC 50

Non-cleavable Linker

DISUCCINIMIDYL SUBERATE(68528-80-3)Related Product Information

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