Congo Red Chemical Properties

Melting point:

>360 °C (lit.)

Density 

0.995 g/mL at 25 °C

vapor pressure 

<1 Pa

storage temp. 

Store at +5°C to +30°C.

solubility 

H2O: soluble10mg/mL

form 

Powder/Solid

pka

4.1(at 25℃)

Colour Index 

22120

color 

Red Brown

PH Range

3(blue)-5.2(red)

Odor

Odorless

PH

6.7 (10g/l, H2O, 20℃)

Water Solubility 

soluble

λmax

497nm, 488nm, 595nm

Merck 

14,2498

BRN 

3894858

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Detecting bacteria,protein folding disorders; treating dermatological disorders,neurodegenerative diseases,Alzheimer’s disease

Major Application

Waveguides, optical sensors, display devices, inks, fertilizer, pesticides, carbohydrates, treatment of pathogen infections, age-related macular degeneration

CAS DataBase Reference

573-58-0(CAS DataBase Reference)

EPA Substance Registry System

Congo Red (573-58-0)

Safety Information

Hazard Codes 

T,Xi

Risk Statements 

45-63-36

Safety Statements 

53-45-37/39-26

WGK Germany 

3

RTECS 

QK1400000

TSCA 

Yes

HS Code 

29215900

Hazardous Substances Data

573-58-0(Hazardous Substances Data)

Toxicity

LD50 i.v. in rats: 190 mg/kg (Richardson, Dillon)

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Congo Red Usage And Synthesis

Artificial dyes

Congo red is aniline dye or coal tar dyes, and it has many types with very wide application. Its drawback is easy to fade by sunlight; aniline blue, bright green, methyl green are more easily fade. Be careful to handle the pH, and avoid direct sunlight when flaking, thus it will keep a few years without fading.
Congo red is an acid dye, a purplish red powder. It is soluble in water and alcohol, turning blue when in acid. It can be used as a dye as well as an indicator. In the production of plant slide, it is often used as a liner agent for hematoxylin or other cell dyes. When used in cytoplasm dyes, it can dye the cytosol or fibrin red. In the process of tissue slicing, it is used to dye nerve axis, elastic fibers, embryonic materials, etc. In addition, Congo red can be used for double staining with hematoxylin; it can also be used for amyloid staining. For it is soluble in water and alcohol, so washing and dehydration treatment should be quick.

Chemical properties

Congo red is red-brown powder, soluble in hot water and the cold water 10 times of its weight; the color of the solution is yellow-red; soluble in ethanol and the solution is orange; slightly soluble in acetone, while almost insoluble in ether. The solution is dark blue when in sulfuric acid, turning to light blue accompanied with the appearance of blue precipitate when diluted.
Its aqueous solution will form a blue floc when in concentrated hydrochloric acid. When added with acetic acid, the precipitate turned blue with a little purple to a precipitate with some red color.  It is insoluble in the concentration of caustic soda solution; susceptible to acid and salt which means even if its absorption of carbon dioxide from the air will make the color blue and dark, while dilute soda solution can restore the original color.
Information on the chemical properties, uses and production methods of artificial dyes Congo Red is compiled and edited by Chemcialbook.

Uses

1. It has been widely used in cotton, viscose dyeing. Because it can easily be transformed into blue when in acid, while there is no appropriate treatment to improve, the usage amount is gradually decreased since the advent of insoluble azo dyes and acid-resistant red 4BS, however, the amount used in the paper industry is quite large. It can also be used as indicators of acid-base, namely Congo red test paper. The measurable range pH is 3.0 (blue)-5.0 (red). It is also used for biological staining, analysis reagents.
2. An indicator frequently used in lab as well as a carcinogenic disperse dyes; used to standard test.
3. Used as an acid-base indicator, adsorption indicator and biological stain, etc.
4. Acid-base indicator, pH 3.0 (blue violet)~5.0 (red); detect the acidity of paper and hydrochloric acid in the gastric juice; test boric acid, cyanide; biological media additives; biological staining, such as embryo slices, plant mucin, cellulose, elastic tissue dyeing.
5. Mainly used for dyeing cotton, hemp, silk and other textile and paper products; it can also be used as an indicator.

Production methods

It is obtained by the coupling with  sodium naphthionate after the diazotization with benzidine, and then by salting out, filtration and drying in the system. The details are as follows: add 48.4 benzidine into 300ml of hydrochloric acid take 48.4g benzidine dissolved in 300ml hydrochloric acid (including 20ml of concentrated hydrochloric acid 20ml), add ice into the solution until the temperature is below 5 ℃; add 10% aqueous solutions prepared by 14.4g sodium nitrite after adding 30ml of concentrated hydrochloric acid.Add the diazonium salt solution slowly into the solution prepared by 150g sodium naphthionate and a little water after diazotization. Add 35g sodium carbonate slowly under the sufficient mixing after 30 minutes' standing, thus appearing an alkaline solution. Heating to 80℃, cooling, filtering, washing with saturated brine and drying can obtain the product. The materials consumption (kg/t) in industrial production are as follows: benzidine (100%) 200, sodium naphthionate (100%) 520, sodium nitrite (industry) 160, hydrochloric acid (31%) 330, soda ash (industrial) 340, fine salt 2500, turkey red oil 9, ethanol 60, sodium acetate 35, sodium sulfate 50.

Description

C.I. Direct red 28(Congo Red) is an odorless,brownish-red powder. Molecular weight =698.72. Meltingpoint =359℃. Soluble in water; solubility =25 g/L at25℃.

Chemical Properties

brown-red powder

Chemical Properties

C.I. Direct red 28(Congo Red) is an odorless, brownish-red powder.

Uses

Dye, medicine (diagnostic aid), indicator, biological stain.

Uses

Congo red is useful for staining and visual detection of amyloid deposits. It inhibits the accumulation of the scrapie-associated, protease-resistant isoform of protein PrP without affecting the metabolism of the normal isoform.

Uses

• Congo red has been used as a histochemical stain for the quantification of amyloid deposits in brain tissues.
• It has been used as a cell wall inhibiting agent in fungus Cryptococcus neoformans.
• It has also been used to study microstructural details in the cell walls of raw potato and fried potato using confocal microscopy.

Definition

ChEBI: An indicator dye that is blue-violet at pH 3.0 and red at pH 5.0.

Biochem/physiol Actions

Congo red is a benzidine-based anionic diazo dye. It is a histological dye which binds to many amyloid proteins and is used for the quantification of amyloid β-peptide aggregation. Congo red also interacts with β-D-glucans, polysaccharides containing continuous β-(1→4)-linked D-glucopyranosyl units and some hemicellulosic galactoglucomannans.

Safety Profile

Human poison by ingestion with cardovascular effects. Experimental poison by intravenous route. An experimental teratogen. Experimental reproductive effects. An eye irritant. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx, SOx, and Na2O

Potential Exposure

It is used as an indicator dye, a biological stain; a diagnostic aid in medicine; and a dye for fabric and paper.

First aid

If this chemical gets into the eyes, remove anycontact lenses at once and irrigate immediately for at least15 min, occasionally lifting upper and lower lids. Seek medical attention immediately. If this chemical contacts theskin, remove contaminated clothing and wash immediatelywith soap and water. Seek medical attention immediately. Ifthis chemical has been inhaled, remove from exposure,begin rescue breathing (using universal precautions, including resuscitation mask) if breathing has stopped and CPR ifheart action has stopped. Transfer promptly to a medicalfacility. When this chemical has been swallowed, get medical attention. If victim is conscious, administer water ormilk. Do not induce vomiting.

storage

Room temperature

Shipping

UN3143 Dyes, solid, toxic, n.o.s. or Dye intermediates, solid, toxic, n.o.s., Hazard Class: 6.1; Labels: 6.1-Poisonous materials, Technical Name Required

Properties and Applications

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	1	3	1	2		2
AATCC	1	3	2	1		3

Purification Methods

Crystallise the dye from aqueous EtOH (1:3). Dry it in air. [Beilstein 6 I 342.]

Incompatibilities

Contact with oxidizers may cause fire and explosion hazard. Incompatible with strong acids; reducing agents.

Congo Red(573-58-0)Related Product Information

• Molasses
• DIRECT FAST RED 3B
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• Sulfamic acid monosodium salt
• Ethylenediaminetetraacetic acid disodium salt
• Nickel bis(sulphamidate)
• Sulfanilic acid
• Sulfamic acid
• Biphenyl
• Sodium N-cyclohexylsulfamate
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• Direct Black 38
• Direct Orange 46
• Direct Yellow 12
• DIRECT BLUE 15
• BRILLIANT YELLOW
• Congo red paper
• Benzidine