4-Methylumbelliferyl beta-D-galactoside Chemical Properties

Melting point:

258-263 °C (dec.)

alpha 

-64 º (c=1, pyridine, after)

Boiling point:

626.9±55.0 °C(Predicted)

Density 

1.522±0.06 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

Soluble in DMSO (40 mg/ml)

pka

12.73±0.70(Predicted)

form 

Powder

color 

White to slightly off-white

Water Solubility 

soluble

Sensitive 

Moisture Sensitive & Hygroscopic

BRN 

94673

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.

InChI

InChI=1/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/s3

InChIKey

YUDPTGPSBJVHCN-ZDHZLMEDNA-N

SMILES

O(C1=CC2OC(C=C(C)C=2C=C1)=O)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O |&1:13,15,18,20,22,r|

CAS DataBase Reference

6160-78-7(CAS DataBase Reference)

Safety Information

Hazard Codes 

T+

Risk Statements 

26/27/28

Safety Statements 

24/25

WGK Germany 

3

F 

3-10-21

HS Code 

29329900

MSDS

• Language:English Provider:7-(beta-D-Galactopyranosyloxy)-4-methyl-2H-1-benzopyran-2-one
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

4-Methylumbelliferyl beta-D-galactoside Usage And Synthesis

Description

4-MUG (6160-78-7) is a versatile fluorogenic substrate for ?-galactosidase1 for use in cell extracts or purified enzyme preparations.1-3? This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm

Chemical Properties

White Powder

Uses

Substrate for fluorometric assay of ?-D-galactosidase

Uses

It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium.

Definition

ChEBI: A beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position.

References

[1] NORMAN H. L. CHIU  Amanda L W. Measuring β-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter[J]. Current protocols in toxicology, 2018, 74 1. DOI:10.1002/cptx.35
[2] KASSIANI KYTIDOU. Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease.[J]. The Journal of Biological Chemistry, 2018: 10042-10058. DOI:10.1074/jbc.ra118.001774
[3] ALICIA HERNÁNDEZ-GUZMÁN. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii[J]. FEBS Open Bio, 2016, 6 11: 1067-1077. DOI:10.1002/2211-5463.12108

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