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4-Methylumbelliferyl beta-D-galactoside

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4-Methylumbelliferyl beta-D-galactoside Basic information

Product Name:
4-Methylumbelliferyl beta-D-galactoside
Synonyms:
  • MUG
  • MU-GAL
  • MUGALACTOSIDE
  • MUGGAL
  • 4-METHYLUMBELLIFERYL-b-D- GALACTOPYRANOSIDE extrapure
  • 4-methylumberiferryl-beta-D-galactopyranoside
  • MUG, MUGA
  • 4-Methyl-7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
CAS:
6160-78-7
MF:
C16H18O8
MW:
338.31
EINECS:
228-185-5
Product Categories:
  • substrate
  • Substrates
  • Enzyme Substrates
  • Fluorescent Labels & Indicators
  • Labeling and Diagnostics Reagents
Mol File:
6160-78-7.mol
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4-Methylumbelliferyl beta-D-galactoside Chemical Properties

Melting point:
258-263 °C (dec.)
alpha 
-64 º (c=1, pyridine, after)
Boiling point:
626.9±55.0 °C(Predicted)
Density 
1.522±0.06 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
Soluble in DMSO (40 mg/ml)
pka
12.73±0.70(Predicted)
form 
Powder
color 
White to slightly off-white
Water Solubility 
soluble
Sensitive 
Moisture Sensitive & Hygroscopic
BRN 
94673
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
CAS DataBase Reference
6160-78-7(CAS DataBase Reference)
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Safety Information

Hazard Codes 
T+
Risk Statements 
26/27/28
Safety Statements 
24/25
WGK Germany 
3
3-10-21
HS Code 
29329900

MSDS

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4-Methylumbelliferyl beta-D-galactoside Usage And Synthesis

Description

4-MUG (6160-78-7) is a versatile fluorogenic substrate for ?-galactosidase1 for use in cell extracts or purified enzyme preparations.1-3? This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm

Chemical Properties

White Powder

Uses

Substrate for fluorometric assay of ?-D-galactosidase

Uses

It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium.

Definition

ChEBI: A beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position.

References

Chiu et al. (2017), Measuring ?-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter; Curr. Protoc. Toxicol., Supp 74 4.44.1 Kytidou et al. (2018), Nicotiana benthamiana α-galactosidase A1,1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease; J. Biol. Chem., 293 10042 Hernandez-Guzman et al. (2016), Purification and characterization of an extracellular ?-glucosidase from Sporothrix schenckii; FEBS Open Bio., 6 1067

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