Cy3 azide Chemical Properties

storage temp. 

-20°C

solubility 

Water, DMSO, DMF

form 

solid

Appearance

Red solid

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26

Cy3 azide Usage And Synthesis

Description

Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

Uses

Azide modified cyanine dye. Can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.
Solubility -DMSO; water; DMF; MeOH
Abs/Em - 553/566 nm
Extinction Coefficient - 151; 000 cm^-1M^-1

reaction suitability

reaction type: click chemistry

Abs/Em Maxima

553/569 nm

Extinction Coefficient

150,000

Microscopy Laser Line

532 or 555 nm

Flow Cytometry Laser Line

532, 555 or 568 nm

Spectrally Similar Dyes

Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549

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