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Acid Blue 83

Basic information Safety Supplier Related

Acid Blue 83 Basic information

Product Name:
Acid Blue 83
Synonyms:
  • SERVA BLUE R
  • SERVA BLUE R STAINING SOLUTION
  • SOLAR CYANINE 6B
  • R STANDS FOR ''REDDISH''
  • PAGE BLUE 83
  • Coomassie brilliant R250
  • Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl) amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino ]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]- N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt
  • Brilliant Blue 6B
CAS:
6104-59-2
MF:
C45H46N3NaO7S2
MW:
827.99
EINECS:
228-060-5
Product Categories:
  • Dyes and Pigments
  • Organics
  • Biochemistry
  • Reagents for Electrophoresis
Mol File:
6104-59-2.mol
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Acid Blue 83 Chemical Properties

Melting point:
174-180 °C
vapor density 
28.5 (vs air)
Flash point:
14 °C
storage temp. 
room temp
solubility 
H2O: soluble10mg/mL
Colour Index 
42660
form 
Powder/Solid
color 
Red Brown
Water Solubility 
Soluble in water.
BRN 
5718025
Stability:
Light Sensitive
InChIKey
NKLPQNGYXWVELD-UHFFFAOYSA-M
CAS DataBase Reference
6104-59-2(CAS DataBase Reference)
EPA Substance Registry System
C.I. Acid Blue 83 (6104-59-2)
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Safety Information

Hazard Codes 
Risk Statements 
Safety Statements 
WGK Germany 
3
TSCA 
Yes
HS Code 
32041200

MSDS

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Acid Blue 83 Usage And Synthesis

Chemical Properties

dark bordeaux to dark purplish-brown cryst. powder

Uses

Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.

Uses

Acid Blue 83 is a dye commonly used in laboratories or quantify proteins.

Preparation

p-Chlorobenzaldehyde?(1 Moore) and 3-(Ethyl(phenyl)amino)methyl)benzenesulfonic acid (2 Moore) condensation, and heavy chromate salt-oxalic acid oxidation its product, again with 4-Ethoxybenzenamine condensation.

Biochem/physiol Actions

Brilliant Blue R is a methanol based stain. It can also stain agarose gels. It consists of methanol and acetic acid.

storage

Room temperature

Properties and Applications

blue. Dark blue powder. Not soluble in cold water, slightly soluble in hot water and ethanol for light blue. The strong sulfuric acid for orange red, diluted into blue. In water solution to join sodium hydroxide purple precipitation. Used for wool, silk, polyamide fiber fabric dyeing and printing directly, can also be used for leather and feathers dyeing.

Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater
Fading Stain Fading Stain Fading Stain
ISO 3 4 4 4 1 1-2 4 4-5
AATCC 2-3 3-4 3-4 4-5 1 1-2 4-5 4-5

Acid Blue 83Supplier

Guangzhou City Haizhu District Zhenghong Co.Ltd. Gold
Tel
020-82550624 13016020539
Email
2336504938@qq.com
BIOISCO(Lianyungang, Jiangsu) Biotechnology Co., Ltd Gold
Tel
18082049515
Email
2757382053@qq.com
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Email
jkinfo@jkchemical.com
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
021-61259108 18621169109
Email
market03@meryer.com
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Email
3bsc@sina.com