Hematoxylin Chemical Properties

Melting point:

200 °C (dec.)(lit.)

Boiling point:

363.32°C (rough estimate)

Density 

1.2514 (rough estimate)

vapor pressure 

0-0Pa at 20-25℃

refractive index 

1.4600 (estimate)

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

solubility 

Soluble in 95% ethanol(1 mg/mL).

Colour Index 

75290

pka

6.7(at 25℃)

form 

Powder/Solid

color 

Yellow to Brown

PH Range

Red (0.0) to yellow (1.0);Pale yellow (5.0) to violet (6.0)

Water Solubility 

SOLUBLE IN HOT WATER

Sensitive 

Light Sensitive

λmax

292nm, 445nm, 560nm

Merck 

14,4637

BRN 

91399

Stability:

Stable, but may discolour on exposure to light. Incompatible with strong oxidizing agents.

Major Application

Plasma displays, textiles, hair dyes, identifying fresh and stale rice, diag-nosing cancer progression, detecting nosing cancer progression, cervical disease, central nervous system malfunctions, detecting genes, breast cancer, collagen in a tissue sample, apoptosis, demyelinating diseases, antigens, treatment of age-related macular degeneration, burns, prostate cancer, diabetesand obesity, viral diseases, neoplasms, peripheral neural and vascular ailments, skin disorders, biotechnological applications, reference standard materials for cytology, histology andimmunohistochemistry

InChIKey

WZUVPPKBWHMQCE-UHFFFAOYSA-N

LogP

0.3 at 30℃ and pH6.9

CAS DataBase Reference

517-28-2

EPA Substance Registry System

Hematoxylin (517-28-2)

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

22-36/37/38

Safety Statements 

26-36

WGK Germany 

2

RTECS 

MH7875000

TSCA 

Yes

HS Code 

32030019

Hazardous Substances Data

517-28-2(Hazardous Substances Data)

MSDS

• Language:English Provider:7,11b-Dihydrobenz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6H)-pentol
• Language:English Provider:SigmaAldrich

Hematoxylin Usage And Synthesis

Chemical Properties

light brown crystals or powder

Uses

Colorant in inks, biological stains.

Uses

Hematoxylin and eosin together make up Hematoxylin and eosin stain, one of the most commonly used stains in histology. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminum-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.

Definition

ChEBI: (+)-haematoxylin is a haematoxylin. It is an enantiomer of a (-)-haematoxylin.

General Description

White to yellowish crystals that redden on exposure to light.

Air & Water Reactions

Soluble in hot water, slightly water soluble in cold .

Reactivity Profile

Sensitive to light. .

Hazard

May be carcinogenic.

storage

Store at RT

Purification Methods

Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.]

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