Basic information Defination Safety Supplier Related
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EC 3.4.21.14

Basic information Defination Safety Supplier Related

EC 3.4.21.14 Basic information

Product Name:
EC 3.4.21.14
Synonyms:
  • SUBTILISIN A
  • PROTEASE ALKALINE
  • e.c.3.4.4.16
  • maxatase
  • nagarse
  • sp266
  • subtilisinbpn’
  • subtilisincarlsburg
CAS:
9014-01-1
MF:
Unspecified
MW:
0
EINECS:
232-752-2
Product Categories:
  • 25kg/drum
  • Hydrolases
  • Specialty Enzymes
Mol File:
Mol File
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EC 3.4.21.14 Chemical Properties

Density 
1.3
vapor pressure 
0Pa at 25℃
storage temp. 
2-8°C
form 
powder
color 
white
Water Solubility 
100g/L at 25℃
Stability:
store cold
LogP
-3.1 at 25℃
CAS DataBase Reference
9014-01-1
EPA Substance Registry System
Subtilisin (9014-01-1)
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Safety Information

Hazard Codes 
Xn,N
Risk Statements 
37/38-41-42-36/37/38-50-22
Safety Statements 
23-24-26-36/37/39-22-36/37-61-45-39
RIDADR 
3082
WGK Germany 
2
RTECS 
UK9540000
3-10
HS Code 
35079090
Hazardous Substances Data
9014-01-1(Hazardous Substances Data)
Toxicity
LD50 orl-rat: 3700 mg/kg FCTXAV 7,581,69
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EC 3.4.21.14 Usage And Synthesis

Defination

Proteases (EC 3.4.21.62) belong to the class of enzymes known as hydrolases, which catalyze hydrolysis of various bonds in presence of water. Proteases are also referred to as Peptidases or Proteinases. Proteases catalyze proteolysis of peptide bonds in polypeptides, proteins and selective hydrolysis of carboxylic esters and amino esters. There are different classes of Proteases, i.e. serine, threonine, cysteine, aspartate, glutamic acid and metallo – proteases.

Chemical Properties

These are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids

Uses

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Uses

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.

Uses

Protease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.

General Description

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.

Safety Profile

Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.

Potential Exposure

These commercial proteolytic enzymes are used in laundry detergent formulations

Purification Methods

This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]

Incompatibilities

Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.

EC 3.4.21.14Supplier

Shanghai JONLN Reagent Co., Ltd. Gold
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Shanghai Yingxin laboratory equipment Co., Ltd Gold
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021-021-59178156 15300768757
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future industrial shanghai co., ltd
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BeiJing Hwrk Chemicals Limted
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0757-86329057 18501085097
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Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Email
zhangsn@titansci.com