ALDOLASE Chemical Properties

storage temp. 

-20°C

form 

powder

color 

white

biological source

rabbit muscle

Specific Activity

10-20units/mg protein

EPA Substance Registry System

Aldolase, fructose diphosphate (9024-52-6)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36-24/25-22

WGK Germany 

3

F 

10-21

MSDS

• Language:English Provider:SigmaAldrich

ALDOLASE Usage And Synthesis

Chemical Properties

White needle-like crystals or lyophilized powder, soluble in water, pI 6.1, optimum pH 7.0. Stability: dry powder can be stored for one year at 4 ℃; the commercial product is a suspension of 3.2 mol/L ammonium sulphate solution, which can be stable for six months at 4 ℃. Inhibitors are metal ions (Cu2+, Ag+, Zn2+), iodine, o-phenanthroline, adenosine nucleoside phosphate (ATP> ADP> AMP), pyridoxal phosphate, D-arachidonitol-1,5-bisphosphate (KI=1.5 μmol/L), urea (4 mol/L) N-bromide acetoacethanol ammonium. Enzyme reaction: D-fructose-1,6-bisphosphate ═dihydroxyacetone phosphate + D-glyceraldehyde-3-phosphate.

Uses

Aldolase from rabbit muscle has been used:

• in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay
• as a standard in the characterization of metabolic enzymes from glaucomatous tissues
• in fructose 2,6-bisphosphate assay of human cell lines

Uses

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts.

Definition

An enzyme present in muscle involved in glycogenolysis and anaerobic glycolysis. It catalyzes production of dihydroxyacetone phosphate and phosphoglyceric aldehyde from fructose-1,6-diphosphate.

General Description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.

Biochem/physiol Actions

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics. It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).

ALDOLASE(9024-52-6)Related Product Information

• 2,4-PENTANEDIONE, SILVER DERIVATIVE
• COBALT(II) ACETYLACETONATE
• METHYL ISOCYANOACETATE
• DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
• 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE
• Ferric acetylacetonate
• TERT-BUTYL ISOCYANIDE
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III)
• Tosylmethyl isocyanide
• TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
• Ethyl isocyanoacetate
• Cupric acetylacetonate
• Aluminum acetylacetonate
• Tris(2,4-pentanedionato)chroMiuM(III)
• PHENYLSELENOL
• N-BUTYLISOCYANIDE
• SALCOMINE
• Benzyl isocyanide