Phosphatase, Acid Chemical Properties

Density 

1.00 g/mL at 20 °C

refractive index 

1.4439

storage temp. 

2-8°C

form 

suspension

color 

white

biological source

rabbit

Water Solubility 

3.2 M ammonium sulfate, pH 6.0, containing 1% BSA: soluble
6% BSA in water: soluble

Specific Activity

864U/mg

CAS DataBase Reference

9001-77-8

EPA Substance Registry System

Phosphatase, acid (9001-77-8)

Safety Information

Hazard Codes 

B,T,F

Risk Statements 

45-11-20/21/22-39/23/24/25-35-22

Safety Statements 

24/25-45-36/37-53-36/37/39-26

RIDADR 

UN 3316 9/PG 3

WGK Germany 

3

F 

3-10

HS Code 

3507.90.7000

MSDS

• Language:English Provider:SigmaAldrich

Phosphatase, Acid Usage And Synthesis

Chemical Properties

White lyophilized powder, soluble in water, optimum pH less than 7.0, with three isoenzymes EI, EII, EIII, optimum pH 5.5, 4.5, 4.0; highest activity against pyrophosphate. Stability: the enzyme solution can be stored in the presence of toluene at 10℃ for 5 months with unchanged vigor. Inhibitors are orthophosphate, molybdate and fluoride. Can promote the blood phosphate ester free inorganic phosphate, enzyme reaction: orthophosphoric acid monoester + H2O ═ alcohol + orthophosphate.

Uses

Acid phosphatase from potato has been used in a study to assess the potential allergenicity of novel gene products. It has also been used in a study to remove eight phosphate groups from casein at a pH of 7.0.

Uses

Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.

Definition

Wheat germ acid phosphatase (WGAP; Phosphatase, Acid from wheat germ) is a commercial preparation of partially purified protein commonly used in laboratory settings for non-specific enzymatic dephosphorylation.

General Description

Orthophosphoric-monoester phosphohydrolase (acid optimum).
Acid phosphatase is a non-specific phosphomonoesterase, found in the cytoplasmic and cell wall sections of swelling potato tubers. It possesses several acidic amino acid residues. It is found to be present in up to six isoforms with varying degree of glycosylation. The major potato isoform is a homodimer having a molar mass of 96kDa.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.

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