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Phenolphthalein diphosphate

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Phenolphthalein diphosphate Basic information

Product Name:
Phenolphthalein diphosphate
Synonyms:
  • PHENOLPHTHALEIN DIPHOSPHATE TETRASODIUM SALT HYDRATE
  • PHENOLPHTHALEIN DIPHOSPHATE
  • 3,3-bis[4-(phosphonooxy)phenyl]-1(3h)-isobenzofuranon
  • 3 3-BIS(P-HYDROXYPHENYL)PHTHALIDE DIPHOSPHATE
  • phenolphthalein phosphate
  • PHENOLPHTHALEIN DIPHOSPHATE FREE ACID
  • Phenolphthalein diphosphate monopyridine salt
  • Phenolphthalein bisphosphate
CAS:
2090-82-6
MF:
C20H16O10P2
MW:
478.28
EINECS:
218-241-7
Mol File:
2090-82-6.mol
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Phenolphthalein diphosphate Chemical Properties

Melting point:
198 °C
Boiling point:
762.1±70.0 °C(Predicted)
Density 
1.671±0.06 g/cm3(Predicted)
storage temp. 
−20°C
form 
solid
pka
0.88±0.30(Predicted)
Water Solubility 
NEGLIGIBLE
CAS DataBase Reference
2090-82-6
EPA Substance Registry System
1(3H)-Isobenzofuranone, 3,3-bis[4-(phosphonooxy)phenyl]- (2090-82-6)
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Safety Information

Safety Statements 
24/25
WGK Germany 
3
HS Code 
29322985

MSDS

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Phenolphthalein diphosphate Usage And Synthesis

Uses

The most commonly used reagents for determining acid phosphatase are phenolphthalein diphosphate or its tetrasodium salt, thymolphthalein disodium monophosphate, and naphthylamine disodium monophosphate. These substances are classic phosphate derivatives used to determine pH values and are usually used in sodium salt form. Among these reagents, phenolphthalein diphosphate or its tetrasodium salt is more effective, easier to observe, less expensive than other reagents, and has low toxicity of the phenolphthalein released into the medium.

Chemical Properties

SLIGHTLY YELLOW FINE CRYSTALLINE POWDER

Enzyme inhibitor

This water-soluble reagent (FW = 491.26 g/mol), also known as phenolphthalein diphosphate and phenolphthalein phosphate, is an alternative substrate for phosphomonoesterases (e.g., alkaline phosphatase), as is the monophosphate. Hydrolysis of the phosphate group generates free phenolphthalein which is red in alkaline conditions. Target(s): inositol 1,4,5-trisphosphate receptors, weakly inhibited; phosphoprotein phosphatase.

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