4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride Chemical Properties

Melting point:

175-177 °C

storage temp. 

2-8°C

solubility 

H2O: soluble50mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)

form 

Powder

color 

White

PH

pH3.0～5.0 (10g/L, 25℃)

Water Solubility 

10 g/L (20 ºC)

Sensitive 

Moisture Sensitive

BRN 

7604627

Stability:

Stable for 1 year from date of purchase as supplied. Solutions at pH <7 are stable for up to 6 months if stored in the refrigerator

InChIKey

WRDABNWSWOHGMS-UHFFFAOYSA-N

CAS DataBase Reference

30827-99-7(CAS DataBase Reference)

Safety Information

Hazard Codes 

C,Xi

Risk Statements 

34-36/37/38

Safety Statements 

45-36/37/39-26-36

RIDADR 

UN 3261

WGK Germany 

3

Hazard Note 

Corrosive

HazardClass 

8

PackingGroup 

II

HS Code 

29214990

MSDS

• Language:English Provider:ACROS

4-(2-Aminoethyl)benzenesulfonylfluoride hydrochloride Usage And Synthesis

Description

AEBSF is a water soluble, irreversible, broad spectrum inhibitor of serine proteases, including trypsin, chymotrypsin, plasmin, thrombin, and kallikreins. AEBSF can also prevent the activation of the ROS generator, NADPH oxidase. At 10-50 μg, AEBSF can attenuate airway inflammation in a mouse model of airway allergy. AEBSF maintains stability in slightly acidic aqueous solutions and serves as a nontoxic alternative to the organophosphate inhibitors, PMSF and DFP.

Chemical Properties

white powder

Uses

AEBSF is extensively used in studies aiming to describe cholesterol regulatory genes due to its potent ability to inhibit Site-1-protease (S1P). This serine protease, located in the Golgi apparatus, is responsible for activating the sterol regulatory element-binding proteins. By selectively inhibiting S1P, AEBSF can be used to characterize the downstream result of SREBP inhibition and its influence on cholesterol regulation.

Uses

Recommended concentrations for use are 0.1-1 mM.

General Description

Pefabloc^? SC is a trypsin inhibitor, which can be used in the synthesis of its arginine-like analogue, 4-(2-guanidinoethyl)benzenesulfonyl fluoride.

Biochem/physiol Actions

Irreversible serine protease inhibitor. Inhibition constants are similar to those of PMSF and DFP. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25?mM.

storage

Store at -20°C

References

1) Powers?et al. (2002),?Irreversible inhibitors of serine, cysteine and threonine proteases; Chem. Rev.,?102?4639 2) Diatchuk?et al.?(1997),?Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds; J. Biol. Chem.,?272?13292

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