Sulfo-SANPAH Chemical Properties

form 

solid

color 

Bright orange-red

InChI

InChI=1S/C16H18N6O9S/c17-20-19-10-5-6-11(12(8-10)22(26)27)18-7-3-1-2-4-15(24)31-21-14(23)9-13(16(21)25)32(28,29)30/h5-6,8,13,18H,1-4,7,9H2,(H,28,29,30)

InChIKey

UPNUQQDXHCUWSG-UHFFFAOYSA-N

SMILES

O(N1C(CC(S(O)(=O)=O)C1=O)=O)C(=O)CCCCCNC1C=CC(N=[N+]=[N-])=CC=1N(=O)=O

Safety Information

HS Code 

2921490090

Sulfo-SANPAH Usage And Synthesis

Description

Sulfo-SANPAH crosslinker (N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate) is a water-soluble, heterobifunctional protein crosslinker with a spacer arm length of 18.2 angstroms. This reagent contains an NHS ester that reacts with primary amines and a nitrophenylazide group that is photoreactive at 320-350 nm toward amino groups. Sulfo-SANPAH commonly enhances Western blot experiments; likewise, Sulfo-SANPAH activated polyacrylamide (PAA) gel crosslinked to fibronectin or collagen matrix (ECM) is used in tissue culture studies.

Uses

Sulfo-sanpah is used as a tether between extracellular matrix protein and substrate.