CHICAGO SKY BLUE 6B Chemical Properties

storage temp. 

room temp

solubility 

DMSO (Very Slightly, Sonicated, Heated), Methanol (Very Slightly, Heated), Water

form 

powder

Colour Index 

24410

color 

Dark Blue to Very Dark Blue

Water Solubility 

Soluble in water (1 mg/ml).

Stability:

Stable. Probably combustible. Incompatible with strong oxidizing agents.

CAS DataBase Reference

2610-05-1

EPA Substance Registry System

C.I. Direct Blue 1, tetrasodium salt (2610-05-1)

Safety Information

Hazard Codes 

Xn,T

Risk Statements 

20/21/22-45

Safety Statements 

22-36-45-53

RIDADR 

3143

WGK Germany 

3

RTECS 

QJ6430000

TSCA 

Yes

Hazardous Substances Data

2610-05-1(Hazardous Substances Data)

MSDS

• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

CHICAGO SKY BLUE 6B Usage And Synthesis

Chemical Properties

greenish blue powder

Uses

Competitive glutamate uptake inhibitor

Uses

Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. It has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.

Preparation

3,3′-Dimethoxylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.

General Description

Bright greenish-blue solid or dark blue powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Slightly soluble in water.

Reactivity Profile

CHICAGO SKY BLUE 6B is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Cleavage of the diazo group by reducing agents may lead to benzidine production.

Fire Hazard

Flash point data for CHICAGO SKY BLUE 6B are not available; however, CHICAGO SKY BLUE 6B is probably combustible.

Biological Activity

Potent inhibitor of L-glutamate uptake into synaptic vesicles.

Biochem/physiol Actions

Chicago Sky Blue 6B is a large organic acid, structurally related to glutamate, that is a potent and efficient competitive inhibitor of vesicular glutamate uptake.

Safety Profile

An experimental teratogen. Other experimental reproductive effects. Mutation data reported. When heated to decomposition it emits very toxic fumes of NOx Na2O, and SOx

storage

Store at RT

Properties and Applications

colourful green light blue. Blue powder. Soluble in water solution for bright blue, solubility of 50 g/L (80 ℃), slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid solution for blue-ray green, blue for diluted; In nitric acid solution for red light purple. The dye solution to join 10% no change sulfuric acid; Add thick sodium hydroxide solution for red light blue. Cellulose fiber of dyeing, the dye well after sex is very good, in 60 ~ 80 ℃ when supreme affinity, discharge sex is very good. In dyeing of copper (colored light turned green, a slight change in iron ion. Mainly used for cotton, viscose and other cellulose fibers, silk, nylon, rayon/nylon blend fabric, paper and biological stain can also be used for cotton and viscose fabric is mainly used for direct printing of cotton, rayon and other cellulose fiber, silk, nylon, rayon / nylon blend fabric, paper and biological stain can also be used for cotton and viscose fabric direct printing.

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	5	4	1	2		2
AATCC	5	1	1	3-4		1

Purification Methods

Free it from other electrolytes by adding aqueous sodium acetate to a boiling solution of the dye in distilled water. After standing, the salted-out dye is filtered on a Büchner funnel, the process being repeated several times. Finally, the precipitated dye is boiled several times with absolute EtOH to wash out any sodium acetate, then dried (as the sodium salt) at 105o. [McGregor et al. Trans Faraday Soc 58 1045 1962, Beilstein 16 II 259.]

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