5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties

Melting point:

240-245 °C (dec.)(lit.)

Boiling point:

671.9±55.0 °C(Predicted)

Density 

1.67

refractive index 

1.6900 (estimate)

storage temp. 

Keep in dark place,Sealed in dry,Room Temperature

form 

Yellow solid

pka

1.57±0.25(Predicted)

color 

Pale Yellow to Light Yellow

Water Solubility 

Soluble in water and ethanol.

Merck 

14,3551

BRN 

1896821

Stability:

Stable. Incompatible with strong bases, strong oxidizing agents.

InChIKey

KIUMMUBSPKGMOY-UHFFFAOYSA-N

CAS DataBase Reference

69-78-3(CAS DataBase Reference)

EPA Substance Registry System

Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

RTECS 

DG9650000

F 

10

TSCA 

Yes

HS Code 

29309070

MSDS

• Language:English Provider:3-Carboxy-4-nitrophenyl disulfide
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis

Chemical Properties

Crystalline

Uses

Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.

Uses

5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.

Definition

ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.

General Description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

5,5′-Dithiobis(2-nitrobenzoic acid)(69-78-3)Related Product Information

• Thiamine disulfide
• LITHIUM PERIODATE
• Folic acid
• Ethyl 2-(Chlorosulfonyl)acetate
• 4-Nitrobenzoic acid
• 2-Nitrobenzoic acid
• 4-Nitrophenyl chloroformate
• Ascoric Acid
• 3,3'-DICARBOXYLIC ACID DIPHENYL DISULFIDE
• 4,4'-Dithiodianiline
• METHYL PHENYL DISULFIDE
• 4,4'-Dinitrodiphenyl disulfide
• 2-Nitrobenzaldehyde
• DIPHENYL DISULFIDE
• 4-NITROTHIOPHENOL
• 2-NITROSOTOLUENE
• 2-Nitrobenzyl alcohol
• Thiobenzoic acid