Lipid Hydroperoxide (LPO) Assay Kit (96 well) Usage And Synthesis

Description

Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders. Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is available in two formats: 100 dtn, which is designed to be read using a single-tube spectrophotometer, and 96 wells, which is designed to be read using a 96-well microplate reader. The 96 well kit contains a special glass plate which is resistant to organic solvents used in the assay; this is the only difference between the two kit types.

References

[1] C E CROSS. Oxygen radicals and human disease.[J]. Annals of Internal Medicine, 1987, 107 4: 526-545. DOI: 10.7326/0003-4819-107-4-526
[2] HALLIWELL B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans.[J]. Free Radical Research, 1996, 25 1: 57-74. DOI: 10.3109/10715769609145656
[3] NED A. PORTER  Karen A M  Sarah E Caldwell. Mechanisms of free radical oxidation of unsaturated lipids[J]. Lipids, 1995, 30 4: 277-290. DOI: 10.1007/bf02536034
[4] M W ROOMI  C Y H. Some reactions of sterculic and malvalic acids. A new source of malvalic acid.[J]. Canadian journal of biochemistry, 1970, 48 7: 759-762. DOI: 10.1139/o70-119
[5] TAKASHI MATSUMURA. N-acetyl-l-tyrosine is an intrinsic triggering factor of mitohormesis in stressed animals.[J]. ACS Applied Nano Materials, 2020: e49211. DOI: 10.15252/embr.201949211
[6] MARTA ESGALHADO . Effect of acute intradialytic strength physical exercise on oxidative stress and inflammatory responses in hemodialysis patients[J]. Kidney Research and Clinical Practice, 2015, 34 1: Pages 35-40. DOI: 10.1016/j.krcp.2015.02.004
[7] CAMILLE FOUSSAL. Activation of catalase by apelin prevents oxidative stress-linked cardiac hypertrophy[J]. FEBS Letters, 2010, 584 11: 2363-2370. DOI: 10.1016/j.febslet.2010.04.025