LEU-LEU METHYL ESTER HYDROBROMIDE Chemical Properties

storage temp. 

−20°C

solubility 

Soluble in DMSO (up to 35 mg/ml) or in Water (up to 40 mg/ml)

form 

solid

color 

White

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or distilled water may be stored at -20°C for up to 1 month.

Safety Information

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

LEU-LEU METHYL ESTER HYDROBROMIDE Usage And Synthesis

Description

Leu-Leu-OMe (16689-14-8) is a lysosomal damaging agent (lysosomotropic). It is condensed into a membranolytic polymer via the transpeptidase action of cathepsin C within lysosomes.1,2 May be used to induce a lysosomal damage model in cells.3,4? LLOMe-induced lysosomal damage elicits ubiquitin puncta formation on lysosomes.1 Induces apoptosis in human mast cells via lysosome destabilization leaving fibroblasts and HEK-293 cells largely resistant.5 Induces lysophagy.4

Uses

H-Leu-Leu-OMe·HBr is a useful research chemical compound used in the preparation of octapeptide derivatives and intermediates.

References

1) Maejima et al. (2013), Autophagy sequesters damaged lysosomes to control lysosomal biogenesis and kidney injury; EMBO J., 32 2336 2) Fujita et al. (2013), Recruitment of the autophagic machinery to endosomes during infection is mediated by ubiquitin; J. Cell Biol., 203 115 3) Chauhan et al. (2016), TRIMS and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-directed Autophagy in Endomembrane Damage Homeostasis; Dev. Cell, 39 13 4) Otomo and Yoshimori (2017), Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery; Methods Mol. Biol. 1594 141 5) Melo et al. (2011), Lysosomal membrane permeabilization induces cell death in human mast cells; Scand. J. Immunol., 74 354

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