Acid Blue 90 Chemical Properties

Melting point:

100 °C

Density 

1.274[at 20℃]

vapor pressure 

0.093Pa at 20℃

Flash point:

11 °C

storage temp. 

2-8°C

solubility 

H2O: soluble1mg/mL

Colour Index 

42655

form 

Solid

color 

Dark blue-violet-brown

Water Solubility 

Soluble in water (50 mg/ml) and ethanol (40 mg/ml).

BRN 

5230822

Stability:

Light Sensitive

InChIKey

JFNOCRSXNFZMNT-UHFFFAOYSA-M

LogP

-2.55 at 20℃

CAS DataBase Reference

6104-58-1(CAS DataBase Reference)

EPA Substance Registry System

C.I. Acid Blue 90 (6104-58-1)

Safety Information

Risk Statements 

Safety Statements 

WGK Germany 

3

TSCA 

Yes

HS Code 

32041200

MSDS

• Language:English Provider:N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfobenzenemethanaminium inner salt monosodium salt
• Language:English Provider:ACROS
• Language:English Provider:SigmaAldrich
• Language:English Provider:ALFA

Acid Blue 90 Usage And Synthesis

Chemical Properties

dark blue-violet-brown crystalline powder

Uses

Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

Preparation

Benzaldehyde ?(1 Moore) and N-ethyl-N-benzyl-3-methylaniline?(2 Moore) condensation, And then sulfonating the product of three acids, and lead oxide, peroxide, and then the reaction with the 4-Ethoxybenzenamine, Benzaldehyde ?sulfonic acid groups on the phenyl ring is substituted anilino-ethoxy.

General Description

Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.

Flammability and Explosibility

Not classified

Biological Activity

brilliant blue g is also a selective inhibitor of the p2x purinoceptor channel p2x7 [1].p2x receptors are membrane ion channels activated in response to the binding of extracellular atp. seven p2x subtypes have been identified. p2x receptors have been involved in in diverse patho- and physiological processes, such as the autonomic nervous system, afferent signalling, chronic pain, and in autocrine loops of endothelial and epithelial cells. the p2x7 receptor plays a prominent role in certain neurologic disorders, such as ischemia-reperfusion injury, alzheimer's disease, spinal cord injury and sensory neuropathies [2].in hek293 cells heterologously expressing human p2x7 receptors, brilliant blue g noncompetitively inhibited rat and human p2x7 receptors with ic50 values of 10 and 200 nm, respectively. the ic50 values for inhibition of the other p2x receptors ranged from 2 to >30 μm; brilliant blue g inhibited the rat and human p2x4 receptors with the ic50 values of >10 and 3.2 μm [1]. subretinal injection of bbg caused retinal cell degeneration at lower concentrations. subretinal injection of bbg (0.25 mg/ml) provided satisfactory biocompatibility [3].

Biochem/physiol Actions

P2X7 purinergic receptor antagonist.

storage

Room temperature

Properties and Applications

bright blue. Dark brown powder, slightly soluble in water, soluble in hot water (bright blue) and ethanol (bright blue). When a blood-red of sulfuric acid, a orange red after diluted. Its water solution and sodium hydroxide for purple. Used for wool and silk dyeing, also can be used for leather dyeing.

Standard	Light Fastness	Soaping		Persperation Fastness	Oxygen bleaching		Fastness to seawater
		Fading	Stain		Fading	Stain	Fading	Stain
ISO	3	3	4-5	3-4	1	1	4	4
AATCC	3	3-4	3-4	4-5	1	1	4-5	4-5

References

[1] jiang l h, mackenzie a b, north r a, et al. brilliant blue g selectively blocks atp-gated rat p2x7 receptors[j]. molecular pharmacology, 2000, 58(1): 82-88.
[2] sperlágh b, vizi e s, wirkner k, et al. p2x 7 receptors in the nervous system[j]. progress in neurobiology, 2006, 78(6): 327-346.
[3] ueno a, hisatomi t, enaida h, et al. biocompatibility of brilliant blue g in a rat model of subretinal injection[j]. retina, 2007, 27(4): 499-504.

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