Aniline Blue Chemical Properties

storage temp. 

Store at +15°C to +30°C.

form 

Powder/Solid

color 

Dark brown-purple crystal with metal luster

EPA Substance Registry System

C.I. Acid Blue 22 (28631-66-5)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

37/39-26-36

WGK Germany 

2

HS Code 

32041200

MSDS

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Aniline Blue Usage And Synthesis

Chemical Properties

Aniline Blue is a dark brownish-purple crystalline powder. It is soluble and used as a biological stain in fluorescent microscopy, appearing yellow-green after excitation with violet light.

Uses

Aniline Blue is a biological stain used for staining collagen and as a fluorescent stain for glycogen. It can also be used to stain collagen fibers in tissue sections using Masson's trichrome protocol for staining multiple components. In this method, collagen stains blue.

Definition

ChEBI: Aniline Blue is an organic sodium salt resulting from the formal condensation of NSC 56820 with 2 mol eq. of sodium hydroxide. It is used as a histological stain for collagen and in Mallory's method for connective tissue.

Preparation

C.I. Solvent Blue 3 (C.I. 42775) with strong sulfuric acid three stlfonation, and translated into sodium salt.

Staining Procedures

Aqueous solution, 0.5-1.0%, alternatively dissolved in ethanol, glycerol or lactoglycerol. Glucans are positive.

Properties and Applications

blue. Soluble in cold water and hot water (blue), slightly soluble in alcohol. Meet strong sulfuric acid as red light yellow, diluted into blue, and produce blue precipitation. Its water solution and sodium hydroxide is palm red light. For wool, silk, cotton, paper, leather dyeing. Used for plastic ones coloring of casein, also used in the manufacture of ink. The salt can be used as organic pigments used in rubber color.

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