Dde Biotin-PEG4-Azide Chemical Properties

storage temp. 

2-8°C

Dde Biotin-PEG4-Azide Usage And Synthesis

Description

Dde Biotin-PEG4-azide is useful for introducing a biotin moiety to alkyne-containing biomolecules using Cu(I)-catalyzed Click Chemistry. The hydrophilic spacer arm provides better solubility to the labeled molecules in aqueous media. Dde protecting group allows efficient release of captured biotinylated molecules from streptavidin under mild conditions with hydrazine.

Uses

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a hydrazine-cleavable Dde linker. Typically >90% of captured biomolecules can be efficiently released.

Dde Biotin-PEG4-Azide(1802907-93-2)Related Product Information

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• PC Biotin-PEG3-Azide
• Dde Biotin-PEG4-Picolyl Azide
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• Methyltetrazine-PEG4-Azide
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• Bis-sulfone-PEG3-Azide
• Methyltetrazine-PEG4-NHS Ester
• Methyltetrazine-PEG4-Acid