DBCO-NHS Ester Chemical Properties

Boiling point:

670.2±65.0 °C(Predicted)

Density 

1.43±0.1 g/cm3(Predicted)

storage temp. 

Storage temp. -20°C

solubility 

Soluble in DMSO, DCM, DMF

pka

-0.23±0.20(Predicted)

form 

Solid

color 

White to Off-White

Appearance

White solid

Stability:

Moisture Sensitive

InChI

InChI=1S/C23H18N2O5/c26-20(13-14-23(29)30-25-21(27)11-12-22(25)28)24-15-18-7-2-1-5-16(18)9-10-17-6-3-4-8-19(17)24/h1-8H,11-15H2

InChIKey

XCEBOJWFQSQZKR-UHFFFAOYSA-N

SMILES

C(N1CC2=CC=CC=C2C#CC2=CC=CC=C12)(=O)CCC(=O)ON1C(CCC1=O)=O

Safety Information

HS Code 

29339900

DBCO-NHS Ester Usage And Synthesis

Description

DBCO-NHS Ester is a very popular amine-reactive click chemistry reagent. It can be used to modify an amine-containing molecule in organic solvents (limited solubility in aqueous media). It reacts with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic pH to form covalent bonds. The low mass weight will add minimal spacer to modified molecules. DBCO is commonly used for copper-free Click Chemistry reactions.

Uses

DBCO-NHS Ester is a derivative of DBCO (Dibenzylcyclooctyne), used in Cu-free click chemistry. It useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. Used in DNA-Assisted protein analysis such as proximity ligation and extention assays. Intracellular Imaging.

Uses

DBCO NHS ester contains an NHS carbonate group and a DBCO group. The spacer contains a photocleavable nitrobenzyloxyl group, which can be efficiently cleaved under UV to release the conjugated molecules. DBCO on the other hand readily react with azide bearing biomolecule through a copper-free click reaction instantly and in high yield.
DBCO-NHS Ester is a very popular amine-reactive click chemistry reagent. It can be used to modify an amine-containing molecule in organic solvents (limited solubility in aqueous media). It reacts with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic pH to form covalent bonds. The low mass weight will add minimal spacer to modified molecules. DBCO is commonly used for copper-free Click Chemistry reactions.

IC 50

Cleavable Linker

DBCO-NHS Ester(1353016-71-3)Related Product Information

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• DBCO-PEG1
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• Oleic-DBCO
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• BDP FL DBCO
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• DBCO-(CH2)2-NH2-CO-(CH2)3COOH
• DBCO-(CH2)3-Acid
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• DBCO-C6-NHS ester