4-Methylumbelliferyl-beta-D-glucuronide Chemical Properties

Melting point:

102 °C

alpha 

-105 º (c=0.25, water 30 ºC)

Boiling point:

683.1±55.0 °C(Predicted)

Density 

1.632±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

Methanol (Slightly), Water (Slightly)

pka

2.76±0.70(Predicted)

form 

White crystalline solid

color 

White

Water Solubility 

slightly soluble

Sensitive 

Moisture Sensitive

InChIKey

ARQXEQLMMNGFDU-YXNYVWNSSA-N

CAS DataBase Reference

6160-80-1(CAS DataBase Reference)

EPA Substance Registry System

.beta.-D-Glucopyranosiduronic acid, 4-methyl-2-oxo-2H-1-benzopyran-7-yl (6160-80-1)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

22-40

Safety Statements 

24/25-36/37

WGK Germany 

3

TSCA 

Yes

HS Code 

29329990

MSDS

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4-Methylumbelliferyl-beta-D-glucuronide Usage And Synthesis

Description

4-Methylumbelliferyl-beta-D-glucuronide is a fluorescent substrate of β-glucuronidase. Once cleaved, 4-Methylumbelliferyl-beta-D-glucuronide yields blue fluorescence seen under UV light which is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively. It is ideal for working with Escherichia coli and plant molecular studies.

Chemical Properties

Crystalline

Uses

Substrate for ?-D-glucuronidase assay. MUG is widely used as component of selective microbial culture media.

Uses

4-Methylumbelliferyl β-D-glucuronide dihydrate is a useful building block and a fluorophore.

Uses

4-Methylumbelliferyl-beta-D-glucuronide is a substrate for glucuronidase. Also used as a test for coliform bacteria in water. Fluorescence: max. Abs. 316nm; max. Em. 375nm; e x 10-3: 12

Definition

ChEBI: A beta-D-glucosiduronic acid having a 4-methylumbelliferyl substituent at the anomeric position.

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