FDG Chemical Properties

Melting point:

200-203 °C (dec.)

Boiling point:

974.8±65.0 °C(Predicted)

Density 

1.74±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMSO: soluble

form 

A solid

pka

12.44±0.70(Predicted)

color 

Yellow to orange

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

8

HS Code 

32129000

MSDS

• Language:English Provider:SigmaAldrich

FDG Usage And Synthesis

Uses

Ultra sensitive fluorogenic substrate for ?-galactosidase, used in live cell and tissue (in vivo and in vitro) assays. This fluorescent substrate reagent could be used for monitoring kinetics of hydrolysis.

Uses

For detecting Glycosidases and could be used in ELISAs, FDG substrate has also been used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry

Uses

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

FDG(17817-20-8)Related Product Information

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