TOTAL DIETARY FIBER ASSAY KIT Chemical Properties

storage temp. 

2-8°C

Major Application

food and beverages
general analytical

Safety Information

Hazard Codes 

Xn

Risk Statements 

37/38-41-42-48/20

Safety Statements 

22-23-26-39-45

Storage Class

6.1C - Combustible acute toxic Cat.3
toxic compounds or compounds which causing chronic effects

Hazard Classifications

Acute Tox. 4 Oral
Aquatic Acute 1
Aquatic Chronic 2
Eye Dam. 1
Resp. Sens. 1
Skin Irrit. 2
STOT RE 1 Inhalation
STOT SE 3

TOTAL DIETARY FIBER ASSAY KIT Usage And Synthesis

Uses

This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with he at stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.

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