Flubendazole Chemical Properties

Melting point:

290°C

Density 

1.3720 (estimate)

storage temp. 

2-8°C

solubility 

Practically insoluble in water, in alcohol and in methylene chloride

pka

10.66±0.10(Predicted)

form 

Solid

color 

White to Off-White

Merck 

14,4118

CAS DataBase Reference

31430-15-6(CAS DataBase Reference)

Safety Information

Risk Statements 

22

Safety Statements 

44

WGK Germany 

2

RTECS 

DD6497500

HS Code 

29339900

Toxicity

LD50 in mice, rats, guinea pigs (mg/kg): >2560 orally (Thienpont)

Flubendazole Usage And Synthesis

Description

Flubendazole is a benzimidazole carbamate anthelmintic. It completely eliminates larvae in a mouse model of A. cantonensis infection and exhibits a mean larval reduction of 100% in a T. spiralis infection model when administered at doses of 5 and 50 mg/kg per day, respectively. Flubendazole inhibits mammalian tubulin polymerization (IC₅₀ = 2.5 μM) and inhibits binding of [³H]mebendazole to H. contortus L3 larval tubulin (IC₅₀ = 0.17 μM). Flubendazole also inhibits the proliferation of BT-549, SK-BR-3, MDA-MB-231, and MCF-7 breast cancer cells (IC₅₀s = 0.72, 1.51, 1.75, and 5.51 μM, respectively) and reduces tumor growth in an MDA-MB-231 mouse xenograft model when administered at a dose of 25 mg/kg.

Chemical Properties

White Solid

Originator

Fluvermal,Janssen-Le Brun,France,1980

Uses

An anthelmintic.

Uses

An insectocidal agent.

Uses

VETRANAL^? analytical standard can be used in the liquid chromatography coupled to mass spectrometry (LC-MS/MS) procedure, performed for the analysis of macrocyclic lactones in milk.

Definition

ChEBI: A member of the class of mebendazole in which the benzoyl group is replaced by a p-fluorobenzoyl group. A broad-spectrum anthelmintic, it is used, particularly in veterinary medicine, for the treatment of nematodal infections.

Manufacturing Process

To a stirred and cooled (ice bath) suspension of 25 parts of aluminum chloride in 52 parts of fluorobenzene is added dropwise a solution of 27.5 parts of 4- chloro-3-nitrobenzoyl chloride in 52 parts of fluorobenzene. Upon completion, stirring is continued overnight at room temperature. The reaction mixture is poured onto water and the product is extracted with methylene chloride. The extract is washed successively with sodium hydrogen carbonate solution and water, dried, filtered and evaporated in vacuo. The solid residue is crystallized from 2-propanol, yielding 4-chloro-4'-fluoro-3-nitrobenzophenone; MP 97.9°C.
A mixture of 24.5 parts of 4-chloro-4'-fluoro-3-nitrobenzophenone, 72 parts of methanol, 13 parts of sulfolane and 3.12 parts of ammonia is heated in a sealed tube for 20 hours at 120°C. To the reaction mixture is added successively 50 parts of water and 25 parts of a diluted hydrochloric acid solution and the whole is stirred and refluxed for 5 minutes. The reaction mixture is cooled and the precipitated product is filtered off. It is washed with 2-propanol and recrystallized from 640 parts of toluene, yielding 4-amino-4'- fluoro-3-nitrobenzophenone; MP 199°C.
A mixture of 14.5 parts of 4-amino-4'-fluoro-3-nitrobenzophenone, 160 parts of methanol, 6 parts of concentrated hydrochloric acid solution and 0.5 part of platinum oxide is hydrogenated at normal pressure and at room temperature. After the calculated amount of hydrogen is taken up, hydrogenation is stopped. The catalyst is filtered off and the filtrate is evaporated. The residue is washed with 2-propanol and dried, yielding 3,4-diamino-4'- fluorobenzophenone hydrochloride; MP 226°C to 230.5°C.
A mixture of 89 parts of S-methylisothiourea sulfate, 6.05 parts of methyl chloroformate in 7 parts of water is cooled, and at a temperature of 5°C to 10°C, sodium hydroxide solution 25% is added until pH equals 8. Then there are added successively 6.4 parts of acetic acid, 2.6 parts of sodium acetate and 8.9 parts of 3,4-diamino-4'-fluorobenzophenone hydrochloride and the whole is stirred while heating at 85°C for 45 minutes (during this reaction time, water and 2-propanol is added). The precipitated product is filtered off, washed with methanol and recrystallized from a mixture of 200 parts of acetic acid and 80 parts of methanol, yielding methyl N-[5(6)-p-fluorobenzoyl-2- benzimidazolyl] carbamate; MP > 260°C.

Therapeutic Function

Anthelmintic

General Description

Flubendazol is a broad spectrum anthelmintic, which belongs to the class of compounds known as benzimidazoles. It can be widely used in veterinary and human medicine. It mainly finds applications in deworming poultry, swine, dogs and cats.

Biochem/physiol Actions

Flubendazole, an antithelmintic, has been widely used in treating intestinal parasites. Additionally, Flubendazole has been reported to exert anticancer activities.

Flubendazole(31430-15-6)Related Product Information

• Imidazole
• Florfenicol
• Benzimidazole
• Methyl formate
• Transfluthrin
• Methyl anthranilate
• Methyl benzoate
• 4-Fluorobenzaldehyde
• Methylparaben
• 2-AMINOFLUBENDAZOLE
• (4-Fluorophenyl)(2-hydroxy-1H-benzi
• Flubendazole EP Impurity F
• 3-NITRO-4-CHLORO-4'-FLUOROBENZOPHENONE
• UMF 060
• Flubendazole D3
• Methanone, 1H-benzimidazol-6-yl(4-fluorophenyl)-
• FENFLURAMINE
• Methyl chloroformate