METHOXYRESORUFIN Chemical Properties

Melting point:

≥220 °C(lit.)

Boiling point:

378.8±42.0 °C(Predicted)

Density 

1.33±0.1 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

DMF: soluble

pka

1.42±0.20(Predicted)

form 

Powder

color 

Brown to reddish brown

BRN 

209529

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

3-8-10

MSDS

• Language:English Provider:SigmaAldrich

METHOXYRESORUFIN Usage And Synthesis

Description

Methoxyresorufin is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2. Methyoxyresorufin is O-demethylated by CYP1A1/2, releasing resorufin, which displays excitation/emission maxima of 570/580 nm, respectively. The appearance of resorufin fluorescence can be used to quantify CYP1A1/2 activity.

Uses

Used to differentiate isozymes of cytochrome P-450

Uses

Substrate for Cytochrome P-450. METHOXYRESORUFIN is used for various investigations including the differentiatiation of cytochrome P-450 isozymes.

Uses

Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).

Biochem/physiol Actions

Fluorimetric substrate for cytochrome P450 linked enzymes.

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