Azure B Chemical Properties

Melting point:

205-210 °C (dec.)(lit.)

storage temp. 

-20°C Freezer

solubility 

H2O: soluble1mg/mL

Colour Index 

52010

form 

Powder

color 

Dark green

Water Solubility 

Soluble

ε(extinction coefficient)

≥13000 at 241-247nm
≥36000 at 287-293nm
≥67000 at 638-644nm

λmax

648–655 nm

Merck 

14,6058

BRN 

3922630

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Antimalarial; biofuel cell; medical devices; diagnosis of amyloid accumulation related diseases,diabetes,malignant melanoma; detecting oral cancer,cells,nucleic acids; treating avian influenza virus,nail infection,oral cavity infection

InChIKey

DNDJEIWCTMMZBX-UHFFFAOYSA-N

CAS DataBase Reference

531-55-5(CAS DataBase Reference)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

RTECS 

SO5550000

HS Code 

32129000

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Azure B Usage And Synthesis

Chemical Properties

green crystalline powder

Uses

Used for staining reticulocytes. Azure B is used for preparing azure eosin stains for blood smears.

Uses

Azure B is used for staining semi-thin sections of plant tissue. This dye is also useful in the NCCLS method for blood smears and Lillie's modified Nocht's method for paraffin sections.

Uses

Biological stain.

Definition

ChEBI: An organic chloride salt having 3-(dimethylamino)-7-(methylamino)phenothiazin-5-ium as the counterion. It is used in making azure eosin stains for blood smear staining and is an active metabolite of methylene blue.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.

Purification Methods

Azure B has been twice recrystallised from H2O and dried at 100o/1hour in an oven. [Beilstein 27 III/IV 5151.]

Azure B(531-55-5)Related Product Information

• Polyvinyl chloride
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• METHYLENE BLUE
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• DIMETHYLMETHYLENE BLUE HYDROCHLORIDE
• AZURE II-EOSIN,AZURE II EOSINATE
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