C12-NBD-DIHYDROCERAMIDE
C12-NBD-DIHYDROCERAMIDE Basic information
- Product Name:
- C12-NBD-DIHYDROCERAMIDE
- Synonyms:
-
- N-C12-NBD-L-THREO-DIHYDROSPHINGOSINE, FLUORESCENT
- N-C12-NBD-D-ERYTHRO-DIHYDROSPHINGOSINE, FLUORESCENT
- N-DODECANOYL-NBD-L-THREO-DIHYDROSPHINGOSINE
- N-DODECANOYL-NBD-D-ERYTHRO-DIHYDROSPHINGOSINE
- C12-NBD-DIHYDROCERAMIDE
- MW:
- 0
- Mol File:
- Mol File
C12-NBD-DIHYDROCERAMIDE Usage And Synthesis
Uses
C-12 NBD-dihydro-Ceramide is a fluorescent ceramidase substrate. C-12 NBD-dihydro-Ceramide compound is a fluorescent ceramide analog which contains a saturated bond in the C4-C5 position of the sphingosine backbone. It can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources. C-12 NBD-dihydro-ceramide may exhibit reduced activity in ceramidase assays compared to C-12 NBD ceramide. This is based on the observation that saturation of the C4-C5 sphingosine bond in C16-ceramide results in approximately a 10-fold reduction in efficiency as a substrate for rat brain ceramidase compared to the unsaturated substrate. Yet, experimental characterization of C-12 NBD-dihydro-ceramide as a ceramidase substrate needs to be performed.